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Kit for separating and identifying yersinia enterocolitica as well as preparation and application thereof

A kit and colon technology, applied in the field of identification including a variety of complex biotypes and toxin-producing or non-reaction combination test screening, can solve the problems of unintuitive screening results, complicated identification steps, and high missed detection rate, and achieve color development. The judgment result is intuitive, and the effect of improving timeliness and accuracy

Inactive Publication Date: 2012-12-12
SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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Problems solved by technology

It mainly solves the technical problems of high missed detection rate, complicated identification steps and high cost, and unintuitive screening results existing in the existing isolation methods of Yersinia enterocolitica

Method used

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  • Kit for separating and identifying yersinia enterocolitica as well as preparation and application thereof

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Embodiment Construction

[0032] First, the kit is prepared according to the content of the technical plan, and the user chooses to use different enrichment solutions and selective plates for combined separation according to different samples, and finally uses simple biochemical and specific enzyme-substrate reaction tests to identify the toxin-producing small intestine and colon. Mori bacteria (Table 2).

[0033] 1. Explanation, collection requirements and preprocessing methods of different test materials

[0034] 1) Feces: including human (patients and carriers), poultry (livestock) and other animal feces samples, collect about 1g (thumb size) as freshly as possible, and collect about 1ml of liquid feces, and inoculate-isolate and culture within 1h-2h . If it cannot be detected for a short time, it should be stored in a commercial medium and transported to the laboratory. First use a cotton swab (swab) to pick a sample and directly inoculate it on a selective agar plate (CIN), incubate at 36°C for ...

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Abstract

The invention relates to a method for separating and identifying yersinia enterocolitica belonging to yersinia in enteric pathogenic bacteria, mainly solving the technical difficult problems of high missed detection rate, complex identifying steps, higher cost and insufficiently visualized screened results in the existing method for separating and identifying the yersinia enterocolitica. A kit comprises 1) general type nonselective enrichment broth, 2) 10-time concentrated general type nonselective enrichment broth, 3) yersinia cold enrichment broth, 4) yersinia quick selective enrichment broth, 5) a selective separation plate, 6) an enterotoxigenic yersinia enterocolitica developing agar plate, 7) a yersinia enterocolitica biochemical identification tube formula, 8) a hydrogen sulfide filter paper strip, and 9) an indole filter paper strip. The yersinia enterocolitica is separated and identified by using combination test of typical colonial morphologogy correspondingly growing by using selective enrichment and selective isolation culture medium, and simple biochemistry and specific enzyme-substrate reaction.

Description

technical field [0001] The present invention relates to the separation and identification method of Yersinia enterocolitica of the genus Yersinia among enteropathogenic bacteria, in particular to the typical colony morphology, simple biochemistry and specificity of the growth corresponding to selective enrichment and selective separation medium Kit, preparation and application of enzyme-substrate reaction combination assay for screening and identification of Yersinia enterocolicus including multiple complex biotypes and toxigenic or non-toxigenic serotypes. Background technique [0002] Yersinia enterocolitica is widely distributed in nature, parasitizes or colonizes in wild animals and domestic artiodactyls (especially pigs) (including lymphatic system and circulation, intestinal tract, etc.), and belongs to the currently known animals The pathogenic bacteria that are clearly quarantined for meat-like products are also one of the important pathogenic bacteria that can cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/04C12R1/01
Inventor 许学斌袁政安金汇明
Owner SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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