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Application of semen euporbiae pulveraqtum in preparation of pharmaceuticals for inhibiting OS-732 cell proliferation

A technology of OS-732 and cell proliferation, which is applied in drug combinations, antineoplastic drugs, and pharmaceutical formulations, etc.

Inactive Publication Date: 2012-12-19
NANJING ZHENGLIANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no literature report on its inhibition of osteosarcoma (OS-732) cell proliferation

Method used

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  • Application of semen euporbiae pulveraqtum in preparation of pharmaceuticals for inhibiting OS-732 cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] Experimental Research Data of Qianjinzi Cream Inhibiting the Proliferation of OS-732 Cells

[0009] 1 Experimental materials

[0010] 1.1 Experimental cell lines

[0011] Osteosarcoma (OS-732), from the laboratory cell bank of our company, was routinely cultured in DMEM+10%FBS.

[0012] 1.2 Experimental drugs

[0013] Research drug: Qianjinzi Cream, Shaanxi Kangwei Bioengineering Co., Ltd., batch number: 20110323

[0014] Liquid stock solution: Weigh 100mg of Qianjinzi cream, dissolve it in 5ml of absolute ethanol, filter through a 0.2μm filter, aliquot into a 500μl doff tube, store at -20°C, and filter absolute ethanol through a 0.2μm filter for use in the control group .

[0015] 1.3 Experimental reagents

[0016] DMEM (GIBCO Cat.No.12100-061Lot.No.758137); Fetal Bovine Serum (Zhejiang Tianhang Biotechnology Co., Ltd. Lot.No.100419); NaHCO3 (Shanghai Jiuyi Chemical Reagent Co., Ltd. Cat.No.11810- 033Lot.No.1088387); Trypsin (AMRESCO batch number: 2010 / 04); EDTA ...

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Abstract

The invention discloses an application of semen euporbiae pulveraqtum in preparation of pharmaceuticals for inhibiting OS-732 cell proliferation. A method for preparing semen euporbiae pulveraqtum comprises the following steps of peeling caper euphorbia seeds and preparing clean kernels into cream according to a cream preparation method specified in pharmacopoeia. The semen euporbiae pulveraqtum is acrid in taste, moderate and toxic, is distributed to liver, kidney and large intestine channels, can have the functions of relieving edema through purgation and dispersing swelling, activating blood stagnation and expelling blood stasis, and is used for treating edema, phlegm and fluid retention, indigestion and distension, urinary stoppage and constipation, blood stasis and amenorrhea; and 0.5-1g of semen euporbiae pulveraqtum externally treats stubborn dermatitis and vegetations; and the semen euporbiae pulveraqtum is almost made into pills and powder to be taken, and the semen euporbiae pulveraqtum is proved to be able to inhibit the OS-732 cell proliferation.

Description

technical field [0001] The invention relates to a new application of a Chinese patent medicine, in particular to the application of Qianjinzi Cream in the preparation of a medicine for inhibiting OS-732 cell proliferation. Background technique [0002] Osteosarcoma refers to tumors of the skeletal system, like other organs, from tumors of any histological component or metastatic lesions from other organs. Tumors involving bone can occur in bone cells, hematopoietic components of bone, cartilage, and fibrous or synovial components. It may be related to factors such as excessive bone growth, chronic inflammatory stimulation, genetic factors, special virus infection, poor blood return in bone, and radiation exposure. The patient's age, gender, and site of occurrence are very helpful in the differential diagnosis of benign and malignant osteomas. Bone cancer tends to occur in young people aged 12 to 20, and primary bone tumor is the most common, followed by metastatic bone can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/47A61P35/00
Inventor 不公告发明人
Owner NANJING ZHENGLIANG MEDICAL TECH