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Polysaccharide particle vaccines

A technology of polysaccharides and particles, applied in nanotechnology, carrier-bound antigen/hapten components, nanotechnology, etc.

Inactive Publication Date: 2012-12-19
LIQUIDIA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A disadvantage of using conjugate vaccines is the difficulty of chemically conjugating the antigen to the protein

Method used

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  • Polysaccharide particle vaccines
  • Polysaccharide particle vaccines
  • Polysaccharide particle vaccines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1: Mouse Study 1, Cross-Linked Protein / Polysaccharide Particles

[0079] Prepare solutions of each particle component by dissolving each component in water at the following concentrations: 2 mg / mL polysaccharide, 10 mg / mL CRM 197 , 55mg / mL glycerol and 55mg / mL ovalbumin. The solutions used to prepare the granules were prepared by mixing the individual component solutions per volume given in the table below. These final solutions contained 3 wt% total solids. In all casting solutions, glycerol was 0.9 wt%, ovalbumin was nominally 2 wt% and polysaccharides (PnP4 or PnP14) were 0.075 wt%. Include CRM 197 The solution was 0.075 wt% toxoid.

[0080]

[0081] Using a #3 Mayer rod, the cast solution was coated on 5 mil raw PET and immediately blown with cold air to evaporate the water and form a film of protein, polysaccharide and glycerol. The film was visually transparent and uniform. A Fluorocur mold with a 200nm x 200nm cylindrical cavity was filled by l...

Embodiment 2

[0099] Example 2: Mouse Study 2

[0100] From 2 mg / mL polysaccharide in WFI, 100 mg / mL glycerol in WFI and lyophilized standard grade ovalbumin, lyophilized endotoxin-free (EndoGrade) ovalbumin, one of lyophilized human serum albumin, and isopropanol (IPA) Prepare casting solution. Two different purities of ovalbumin were used to control for potential immune effects of lipopolysaccharide present in standard grade ovalbumin. The final concentrations of the components are as follows:

[0101]

[0102] Using a #3 Mayer rod, coat the cast solution on 5 mil green PET and immediately blow with cold air to evaporate the solvent. A Fluorocur mold with a 200nm x 200nm cylindrical cavity was filled by laminating the mold with a film followed by a heated clamp [Temperature: 210-220°F, Pressure: 60psi, Speed: 2fpm]. The PET was separated from the mold after passing through the heating nip, leaving the mold cavity filled with the film composition. The filled mold was then laminate...

Embodiment 3

[0115] Example 3: Cross-linked protein / polysaccharide particles for other vaccines

[0116] In addition to PS selected from Neumera, Meningococcus, Typhoid, Cell Surface Glycolipids, Glycoproteins, HiB, Staphylococcus, Chlamydia, MenB, Clostridium difficile, Pseudomonas, Streptococcus A and B, ETEC , TB, Shigella, Salmonella typhi, botulinum, pestilence or Burkholderia, the method was carried out in substantially the same manner as described in Example 1 or 2.

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Abstract

Particle compositions are prepared for use as polysaccharide particle vaccines.

Description

Background technique [0001] Conjugate vaccines are usually produced by covalently linking an antigen to a carrier protein. The resulting immunogen is then often administered to bacterial polysaccharides for the prevention of bacterial disease. One disadvantage of using conjugate vaccines is the difficulty in chemically conjugating the antigen to the protein. Thus, substantially conjugate vaccines that do not require direct conjugation of protein to carbohydrate, where the antigen and protein are present in close association with the target cells, would be highly desirable. [0002] via PRINT ? Microparticles and Nanoparticles Manufactured by Liquidia Technologies (Liquidia Technologies, North Carolina) Provides microparticles and nanoparticles with control over their chemical composition, particle size, particle shape, surface functionality, and other physical and chemical characteristics. The use of this technology in the present invention enables the co-packaging of antig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00
CPCA61K2039/55544A61K2039/55555A61K2039/545A61K39/092A61K39/385Y10S977/773
Inventor S.M.雷勒A.墨菲B.胡拜
Owner LIQUIDIA TECH