Semen diluent convenient to count duck sperms and application method thereof

Abstract

The invention discloses semen diluent convenient to count duck sperms and an application method of the semen diluent. The semen diluent is composed of the following components: 40 to 60g of NaHCO3 (sodium bicarbonate), 5 to 15mL of 35% of formaldehyde, 8 to 12g of gentian violet, 5 to 15mL of 96% of alcohol, and normal saline added to 1000mL. The semen after being diluted by the diluent is capable of not only quickly killing the sperms, but also improving definition when a microscopic examination is carried out, so that quantity of the duck sperms is accurately counted, and density of the drake sperms is detected, thereby providing an important reference on use of the drakes, dilution, storage and transportation of semen.

Description

technical field

[0001] The invention belongs to the technical field of duck artificial insemination, and in particular relates to a semen diluent for duck sperm counting and a method for using the same. Background technique

[0002] The evaluation of duck semen quality can identify the quality of semen and determine its utilization value; it is the only means to test the effect of semen dilution, preservation and transportation; it is an indispensable and important link in the process of duck artificial insemination, and the density of duck sperm It is an important indicator to measure the quality of semen. Sperm counting method is an important method for sperm density detection. In order to accurately count the number of sperm, when counting sperm count (blood cell counting method), the semen should be diluted by a multiple of 1:200. Under normal circumstances, only normal saline is used to dilute the semen, which does not kill the sperm. Most of the sperm are still in a s...

Images