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Semen diluent convenient to count duck sperms and application method thereof

A sperm counting and diluting technology, applied in the field of semen diluting solution, can solve problems such as inability to kill sperm, unfavorable sperm counting, inability to accurately measure the density of duck sperm, etc., and achieve the effect of improving clarity

Inactive Publication Date: 2012-12-26
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, only normal saline is used to dilute the semen, which does not kill the sperm. Most of the sperm are still in motion under a 400-fold microscope, which is not conducive to the count of sperm, and thus cannot accurately measure the density of duck sperm.

Method used

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  • Semen diluent convenient to count duck sperms and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Configuration of semen diluent: weigh NaHCO separately 3 Put 40g and 8g of gentian violet in a 250ml beaker, add 5ml of 35% formaldehyde and 96% alcohol, stir with a glass rod, transfer it into a 1000ml volumetric flask after completely dissolving, add normal saline to the mark and shake well.

[0011] Use a pipette gun with a volume of 10 μl to draw 10 μl of semen into the glass, and then use a disposable syringe with a volume of 2ml to draw 2ml of the prepared diluent into the glass, and pipette up and down several times to make it evenly mixed. Use a pipette gun to draw 20 μl of diluted semen and drop it on the edge between the counting plate and the cover glass, so that the semen will automatically infiltrate into the calculation chamber. When the semen is added to the hemocytometer, it must be placed for at least 1 min to allow the sperm to settle for easy counting.

Embodiment 2

[0013] Configuration of semen diluent: weigh NaHCO separately 3 Put 60g and 12g of gentian violet into a 250ml beaker, add 15ml of 35% formaldehyde and 96% alcohol, stir with a glass rod, transfer it into a 1000ml volumetric flask after completely dissolving, add normal saline to the mark and shake well.

[0014] Use a pipette gun with a volume of 10 μl to draw 10 μl of semen into the glass, and then use a disposable syringe with a volume of 2ml to draw 2ml of the prepared diluent into the glass, and pipette up and down several times to make it evenly mixed. Use a pipette gun to draw 20 μl of diluted semen and drop it on the edge between the counting plate and the cover glass, so that the semen will automatically infiltrate into the calculation chamber. When the semen is added to the hemocytometer, it must be placed for at least 1 min to allow the sperm to settle for easy counting.

Embodiment 3

[0016] Configuration of semen diluent: weigh NaHCO separately 3 Put 50g and 10g of gentian violet in a 250ml beaker, add 10ml of 35% formaldehyde and 96% alcohol, stir with a glass rod, transfer it into a 1000ml volumetric flask after completely dissolving, add normal saline to the mark and shake well.

[0017] Use a pipette gun with a volume of 10 μl to draw 10 μl of semen into the glass, and then use a disposable syringe with a volume of 2ml to draw 2ml of the prepared diluent into the glass, and pipette up and down several times to make it evenly mixed. Use a pipette gun to draw 20 μl of diluted semen and drop it on the edge between the counting plate and the cover glass, so that the semen will automatically infiltrate into the calculation chamber. When the semen is added to the hemocytometer, it must be placed for at least 1 min to allow the sperm to settle for easy counting.

[0018] figure 1 It is the state of the sperm under the microscope at 400 times after the dilue...

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Abstract

The invention discloses semen diluent convenient to count duck sperms and an application method of the semen diluent. The semen diluent is composed of the following components: 40 to 60g of NaHCO3 (sodium bicarbonate), 5 to 15mL of 35% of formaldehyde, 8 to 12g of gentian violet, 5 to 15mL of 96% of alcohol, and normal saline added to 1000mL. The semen after being diluted by the diluent is capable of not only quickly killing the sperms, but also improving definition when a microscopic examination is carried out, so that quantity of the duck sperms is accurately counted, and density of the drake sperms is detected, thereby providing an important reference on use of the drakes, dilution, storage and transportation of semen.

Description

technical field [0001] The invention belongs to the technical field of duck artificial insemination, and in particular relates to a semen diluent for duck sperm counting and a method for using the same. Background technique [0002] The evaluation of duck semen quality can identify the quality of semen and determine its utilization value; it is the only means to test the effect of semen dilution, preservation and transportation; it is an indispensable and important link in the process of duck artificial insemination, and the density of duck sperm It is an important indicator to measure the quality of semen. Sperm counting method is an important method for sperm density detection. In order to accurately count the number of sperm, when counting sperm count (blood cell counting method), the semen should be diluted by a multiple of 1:200. Under normal circumstances, only normal saline is used to dilute the semen, which does not kill the sperm. Most of the sperm are still in a s...

Claims

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Application Information

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IPC IPC(8): G01N1/38G01N33/487
Inventor 辛清武郑嫩珠朱志明缪中纬
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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