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A method for rapidly measuring the temperature resistance of phytase

A rapid determination of phytase technology, applied in the direction of color/spectral characteristic measurement, etc., to achieve good repeatability, reduce layer-by-layer sampling error, and avoid interference

Active Publication Date: 2016-01-20
北京昕大洋科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the actual granulation method by measuring the retention rate of phytase in the actual granulation process is not an ideal method to evaluate the temperature tolerance of phytase

Method used

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  • A method for rapidly measuring the temperature resistance of phytase
  • A method for rapidly measuring the temperature resistance of phytase
  • A method for rapidly measuring the temperature resistance of phytase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 carries out multiple repeated determinations to the same high temperature resistant phytase sample

[0036] (1) Test materials and equipment: phytase sample (Xinyang phosphorus coated pellet phytase, batch number 0106021009) (5000U / g) 200g; aluminum foil bag: outer edge length 12cm × width 10cm; water bath ( HH-601A); plastic sealing machine (GK9-69M).

[0037] (2) Determination method of enzyme activity: determined according to the method of GB / T18634-2009.

[0038] (3) Test plan:

[0039] Test conditions: Water bath measurement temperature 85°C, 90°C (Note: The temperature is measured by a calibrated mercury thermometer).

[0040] experiment method:

[0041] (3.1) Take 100g of phytase product and put it into No. 7 plastic sealing bag, use the following calculation formula to calculate the amount of distilled water when the water content is replenished to 18%, which is 14.6g, then accurately weigh the distilled water and add it to the plastic Seal the ...

Embodiment 2

[0053] Embodiment 2 carries out thermotolerance determination to different phytase samples

[0054] (1) Test materials and equipment: two high temperature-resistant phytase samples with a specification of 5000U / g (high temperature resistance A: Xinyang phosphorus high temperature resistant phytase batch number 0311021010; and high temperature resistance B: Xinyang phosphorus Pellet phytase batch number 0208011009;) and a common powdered phytase (Xin Yang phosphorus powdered phytase batch number 0302011008) each 200g; aluminum foil bag: outer edge length 12cm × width 10cm; water bath ( HH-601A); plastic sealing machine (GK9-69M).

[0055] (2) Determination method of enzyme activity: determined according to the method of GB / T18634-2009.

[0056] (3) Test plan:

[0057] Test conditions: Water bath measurement temperature 85°C, 90°C, 95°C (Note: The temperature is measured by a calibrated mercury thermometer).

[0058] experiment method:

[0059] (3.1) Get above-mentioned three ...

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Abstract

The invention provides a method for rapidly determining heat resistance of phytase. Firstly, heat resistance of a phytase product is directly determined by the adoption of the method. One on hand, stratified sampling errors caused by a nonuniform mixing phenomenon generated when the phytase product is added into a feed are greatly reduced; and on the other hand, interference with phytase detection accuracy caused by other substances in the feed is also avoided. Secondly, it is ensured that each sample is uniformly heated by simulation of pelleting temperature and moisture in practical pellet feed production and by a water-bath heating mode. Finally, as sample quantity and operational details are stipulated, it is ensured that determination has good repeatability.

Description

technical field [0001] The invention relates to a method for quickly measuring the temperature-resistant performance of phytase. Background technique [0002] At present, phytase has been widely used in the feed production of monogastric animals, which greatly reduces the cost of feed and the environmental pollution caused by the excretion of phosphorus, and has very significant economic, social and ecological benefits. However, in China's feed industry, more than 70% of the feed is pelleted feed, and the chemical essence of phytase is protein, which is very sensitive to heat, so it is very easy to inactivate during the feed pelleting process. With the continuous progress of modern biotechnology and enzyme engineering technology, the production process of phytase has also made great progress, and various high-temperature resistant phytase products have emerged as the times require, but due to the lack of simple and effective high-temperature performance evaluation methods, m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 郭宝林魏秀莲苏俊兵
Owner 北京昕大洋科技发展有限公司