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Method for rapidly determining heat resistance of phytase

A rapid determination of phytase technology, applied to the measurement of color/spectral characteristics, etc., to achieve good repeatability, reduce layer-by-layer sampling errors, and avoid interference

Active Publication Date: 2013-01-02
北京昕大洋科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the actual granulation method by measuring the retention rate of phytase in the actual granulation process is not an ideal method to evaluate the temperature tolerance of phytase

Method used

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  • Method for rapidly determining heat resistance of phytase
  • Method for rapidly determining heat resistance of phytase
  • Method for rapidly determining heat resistance of phytase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 The same high-temperature resistant phytase sample was repeatedly tested

[0036] (1) Test materials and equipment: phytase sample (Xinyang phosphorus coated pellets phytase, batch number 0106021009) (5000U / g) 200g; aluminum foil bag: outer edge length 12cm×width 10cm; water bath ( HH-601A); Plastic sealing and sealing machine (GK9-69M).

[0037] (2) Enzyme activity determination method: in accordance with GB / T18634-2009 method.

[0038] (3) Test plan:

[0039] Test conditions: The temperature measured in a water bath is 85°C, 90°C (Note: The temperature is measured by a calibrated mercury thermometer).

[0040] experiment method:

[0041] (3.1) Take 100g of phytase product and put it into No. 7 plastic sealing bag, use the following formula to calculate the amount of distilled water when the water content is added to 18% is 14.6g, then accurately weigh the distilled water and add it to the plastic In the sealed bag, seal the mouth of the bag and mix thoroughly. Weigh...

Embodiment 2

[0053] Example 2 Determination of temperature resistance of different phytase samples

[0054] (1) Test materials and equipment: two high temperature resistant phytase samples with specifications of 5000 U / g (High temperature resistant A: Xinyang Phosphorus high temperature resistant granular phytase batch number 0311021010; and High temperature resistant B: Xinyang Phosphorus Pellets phytase batch number 0208011009;) and one ordinary powdered phytase (Xinyang Phosphorus powdered phytase batch number 0302011008) each 200g; aluminum foil bag: outer edge length 12cm×width 10cm; water bath ( HH-601A); Plastic sealing and sealing machine (GK9-69M).

[0055] (2) Enzyme activity determination method: Measure according to GB / T18634-2009 method.

[0056] (3) Test plan:

[0057] Test conditions: The temperature measured in a water bath is 85°C, 90°C, and 95°C (Note: The temperature is measured by a calibrated mercury thermometer).

[0058] experiment method:

[0059] (3.1) Take 150g of the abov...

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Abstract

The invention provides a method for rapidly determining heat resistance of phytase. Firstly, heat resistance of a phytase product is directly determined by the adoption of the method. One on hand, stratified sampling errors caused by a nonuniform mixing phenomenon generated when the phytase product is added into a feed are greatly reduced; and on the other hand, interference with phytase detection accuracy caused by other substances in the feed is also avoided. Secondly, it is ensured that each sample is uniformly heated by simulation of pelleting temperature and moisture in practical pellet feed production and by a water-bath heating mode. Finally, as sample quantity and operational details are stipulated, it is ensured that determination has good repeatability.

Description

Technical field [0001] The invention relates to a method for quickly determining the temperature resistance of phytase. Background technique [0002] At present, phytase has been widely used in the production of monogastric animal feed, which greatly reduces the cost of feed and the environmental pollution caused by excretion of phosphorus, and has very significant economic, social and ecological benefits. However, in China's feed industry, more than 70% of the feed is pelletized feed, and the chemical nature of phytase is protein, which is very sensitive to heat, so it is very easy to be inactivated during feed pelleting. With the continuous progress of modern biotechnology and enzyme engineering technology, phytase production technology has also made great developments, and various high-temperature resistant phytase products have emerged. However, due to the lack of simple and effective high-temperature performance evaluation methods, Users are in a dilemma when choosing high-...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 郭宝林魏秀莲苏俊兵
Owner 北京昕大洋科技发展有限公司