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Simple preparation method of bacillus bacteria powder

A technology of bacillus and bacteria powder, applied in the field of micro-ecological preparations, can solve the problems of complex production process, rapid equipment loss, high application cost, etc., and achieve the effect of low technical difficulty, simple process and promotion of application

Inactive Publication Date: 2013-01-16
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The vacuum freeze-drying method is also one of the most widely used methods in the application of microbial products. Through the vacuum freeze-drying method, micro-ecological products with a high rate of viable bacteria can also be obtained, but the vacuum freeze-drying method has the following problems: high application cost ( 5-8 times that of spray drying), time cost is relatively high (48h / batch), equipment loss is relatively rapid, and the prepared products are assembled together, which needs further crushing, powder making, and poor fluidity
Although these three processing methods are widely used, they all require the addition of expensive drying protective agents or carriers, professional drying equipment, and the production process is relatively complicated, which must be operated by professional personnel.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 Bacillus subtilis bacterial powder simple preparation method

[0023] Use the following medium to ferment Bacillus subtilis CGMCC No.4628: 10g of glucose, 10g of bean cake powder, 5g of sodium chloride, 0.6g of manganese sulfate, and adjust the pH by dilute hydrochloric acid and sodium hydroxide. During the fermentation process, the pH is stable at 7.0, 28 ℃ fermentation for 72 hours.

[0024] After the fermentation is over, mix the fermented liquid and corn cob powder in a weight ratio of 1:1, and after stirring evenly, spread out pancakes on a curved plate with a thickness of 2cm, and place them on a plate rack to dry naturally. When the water content drops to 10%, the bacteria powder of Bacillus subtilis CGMCC No.4628 is obtained. After testing, the bacteria powder contained Bacillus subtilis CGMCC No.4628, and the number of viable bacteria was 2.7×10 9 cfu / g, spore rate 82.3%.

Embodiment 2

[0025] The simple preparation method of embodiment 2 Bacillus licheniformis bacterial powder

[0026] Use the following medium to ferment Bacillus licheniformis CGMCC No.5094: 12g of glucose, 11g of bean cake powder, 4g of sodium chloride, 0.5g of manganese sulfate, and adjust the pH by dilute hydrochloric acid and sodium hydroxide. During the fermentation process, the pH is stable at 7.0, 28 ℃ fermentation for 72 hours.

[0027] After the fermentation is over, mix the fermented liquid and corncob powder at a weight ratio of 1:1.2, and after stirring evenly, spread out pancakes on a curved plate with a thickness of 2.5 cm, and place them on a plate rack to dry naturally. When the water content drops to 15%, the bacteria powder of Bacillus licheniformis CGMCC 5094 is obtained. After testing, the bacteria powder contained Bacillus licheniformis CGMCC 5094, and the number of viable bacteria was 3.2×10 9 cfu / g, spore rate 79.6%.

Embodiment 3

[0028] Embodiment 3 simple preparation method of bacillus pumilus bacterial powder

[0029] Use the following medium to ferment Bacillus pumilus CGMCC No.4756: 10g of glucose, 11g of bean cake powder, 5g of sodium chloride, 0.5g of manganese sulfate, and adjust the pH by dilute hydrochloric acid and sodium hydroxide. During the fermentation process, the pH is stable at 7.0, 28 ℃ fermentation for 72 hours.

[0030] After the fermentation is over, mix the fermented liquid and corncob powder in a weight ratio of 1:1.5, and after stirring evenly, spread out pancakes on a curved plate with a thickness of 3 cm, and place them on a plate rack to dry naturally. When the water content drops to 5%, the bacteria powder of Bacillus pumilus CGMCC No.4756 is obtained. After testing, the bacterial powder contained Bacillus pumilus CGMCC No.4756, and the number of viable bacteria was 2.4×10 9 cfu / g, spore rate 84.6%.

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PUM

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Abstract

The invention discloses a simple preparation method of bacillus bacteria powder. The method comprises the steps of 1) mixing fermented bacillus fermentation liquor and a bacteria liquid adsorbent according to a weight ratio of 1.0 : 1.0-1.5, stirring uniformly; 2) spreading the uniformly-mixed mixture of the fermentation liquor and the bacteria liquid adsorbent with a thickness of 2 cm-3 cm, putting in a ventilated position for naturally drying; and finishing the preparation of the bacteria powder when the water content is reduced to 20% or lower. Microbial ecological agents are prepared directly by employing the bacteria liquid adsorbent to absorb active bacteria and metabolism products thereof in the fermentation liquor and drying. The method is simple in process, has low technical difficulty, omits a thallus separating procedure after the fermentation is finished, and makes the most of beneficial metabolites of probiotics in the fermentation liquor.

Description

technical field [0001] The invention belongs to the technical field of microecological preparations and relates to a simple preparation method for feeding bacillus powder. Background technique [0002] Microecological preparations refer to live bacteria mainly used for animals that contain only live bacteria or bacterial cells and their metabolites, which are isolated from animals inside and outside the body and beneficial to animals according to the theory of microecology. preparation. [0003] The processing methods currently used for probiotics preparations mainly include spray drying, vacuum freeze drying and microcapsule coating. Most of the existing micro-ecological products in the market adopt the spray drying method. The advantage of the drying method is that the processing capacity is large, the obtained microcapsule powder has good fluidity, and the processing process is easy to control. The vacuum freeze-drying method is also one of the most widely used methods ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/00A23L1/16A23L7/109
Inventor 汪攀张军孙晓雯闫轶洁王安如
Owner BEIJING DABEINONG TECH GRP CO LTD
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