Simple preparation method of bacillus bacteria powder
A technology of bacillus and bacteria powder, applied in the field of micro-ecological preparations, can solve the problems of complex production process, rapid equipment loss, high application cost, etc., and achieve the effect of low technical difficulty, simple process and promotion of application
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Embodiment 1
[0022] Embodiment 1 Bacillus subtilis bacterial powder simple preparation method
[0023] Use the following medium to ferment Bacillus subtilis CGMCC No.4628: 10g of glucose, 10g of bean cake powder, 5g of sodium chloride, 0.6g of manganese sulfate, and adjust the pH by dilute hydrochloric acid and sodium hydroxide. During the fermentation process, the pH is stable at 7.0, 28 ℃ fermentation for 72 hours.
[0024] After the fermentation is over, mix the fermented liquid and corn cob powder in a weight ratio of 1:1, and after stirring evenly, spread out pancakes on a curved plate with a thickness of 2cm, and place them on a plate rack to dry naturally. When the water content drops to 10%, the bacteria powder of Bacillus subtilis CGMCC No.4628 is obtained. After testing, the bacteria powder contained Bacillus subtilis CGMCC No.4628, and the number of viable bacteria was 2.7×10 9 cfu / g, spore rate 82.3%.
Embodiment 2
[0025] The simple preparation method of embodiment 2 Bacillus licheniformis bacterial powder
[0026] Use the following medium to ferment Bacillus licheniformis CGMCC No.5094: 12g of glucose, 11g of bean cake powder, 4g of sodium chloride, 0.5g of manganese sulfate, and adjust the pH by dilute hydrochloric acid and sodium hydroxide. During the fermentation process, the pH is stable at 7.0, 28 ℃ fermentation for 72 hours.
[0027] After the fermentation is over, mix the fermented liquid and corncob powder at a weight ratio of 1:1.2, and after stirring evenly, spread out pancakes on a curved plate with a thickness of 2.5 cm, and place them on a plate rack to dry naturally. When the water content drops to 15%, the bacteria powder of Bacillus licheniformis CGMCC 5094 is obtained. After testing, the bacteria powder contained Bacillus licheniformis CGMCC 5094, and the number of viable bacteria was 3.2×10 9 cfu / g, spore rate 79.6%.
Embodiment 3
[0028] Embodiment 3 simple preparation method of bacillus pumilus bacterial powder
[0029] Use the following medium to ferment Bacillus pumilus CGMCC No.4756: 10g of glucose, 11g of bean cake powder, 5g of sodium chloride, 0.5g of manganese sulfate, and adjust the pH by dilute hydrochloric acid and sodium hydroxide. During the fermentation process, the pH is stable at 7.0, 28 ℃ fermentation for 72 hours.
[0030] After the fermentation is over, mix the fermented liquid and corncob powder in a weight ratio of 1:1.5, and after stirring evenly, spread out pancakes on a curved plate with a thickness of 3 cm, and place them on a plate rack to dry naturally. When the water content drops to 5%, the bacteria powder of Bacillus pumilus CGMCC No.4756 is obtained. After testing, the bacterial powder contained Bacillus pumilus CGMCC No.4756, and the number of viable bacteria was 2.4×10 9 cfu / g, spore rate 84.6%.
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