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Human NK/T cell line

A cell line, a human technology, applied in the field of cell lines, can solve the problem of no NK/T cells, etc., and achieve the effect of strong anti-tumor activity

Active Publication Date: 2014-07-02
ZHEJIANG CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Although the NK cells disclosed in the above patents have made some progress in anti-tumor, due to the technical difficulties in the purification and large-scale expansion of NK cells in vitro, there is only one strain of human nature with the preservation number CGMCC NO.2901 in China. Killer cells NKG cells, there is no report on the establishment of human NK / T cell lines

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  • Human NK/T cell line
  • Human NK/T cell line
  • Human NK/T cell line

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Experimental program
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Embodiment Construction

[0020] (1) Establishment of ZQNK-29 cell line

[0021] 1.1 The source of the specimen:

[0022] 10ml of peripheral blood was taken from a male patient with nasal angiocentric NK / T cell lymphoma, anticoagulated with heparin. With the consent of the patient and the informed consent of the family members.

[0023] 1.2 Isolation of peripheral blood mononuclear cells:

[0024] 10ml of peripheral blood was aseptically extracted from the patient, 2ml of which was measured by conventional methods, and the T cell immune subsets were determined by flow cytometry. The results were: CD25=60%, CD19=1%, CD4=13%, CD8=3 %, CD56=81%, CD3=16%, CD44=71%. The remaining 8ml of peripheral blood was diluted with an equal amount of sterile Hank’s solution, mixed evenly, and subjected to density gradient centrifugation with lymphocyte separation medium at 2000 rpm for 20 minutes at room temperature at 20 degrees. Aspirate the mononuclear cells in the middle layer, wash twice with Hank's solution, ...

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Abstract

The invention discloses a human NK / T cell line, which is named as human NK / T cell line ZQNK-29 and is preserved in China Center for Type Culture Collection (CCTCC) in Wuhan university, and the preservation number is CCTCC C201278. The human NK / T cell line can realize the long-time transfer of culture, the mass amplification is realized, in addition, higher anti-tumor activity is realized, and a novel experiment model is provided for researchers to deeply developing nature killing cell anti-tumor acting mechanism and clinic adoption immunotherapy.

Description

technical field [0001] The invention belongs to a cell line, in particular to a human NK / T cell line ZQNK-29. Background technique [0002] 30 years ago, scientists discovered that there are natural killer cells (natural killer cells, NK) in white blood cells, which are important immune cells in the body. Since the killing activity of NK cells is not limited by MHC and does not depend on antibodies, it is called natural killer cells. . NK cells are not only related to anti-tumor, anti-viral infection and immune regulation, but also participate in the occurrence of hypersensitivity and autoimmune diseases in some cases. Since then, scientists have studied the specific function of natural killer cells, theorizing that natural killer cells activate other white blood cells, such as T lymphocytes and macrophages, and activate immune responses against various infections. [0003] NK cells have always been considered as the body's best weapon to kill cancer cells and infectious p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0783C12R1/91
Inventor 钱丽娟顾琳慧牟瀚舟凌志强冯建国杜灵彬凌雨田姜志明孙文勇孔祥鸣朱赤红许沈华苏丹毛伟敏
Owner ZHEJIANG CANCER HOSPITAL