Chlorella mutant strain and application thereof

A technology of mutant strains and chlorella, which is applied in the direction of mutant preparation, single-cell algae, and methods of using algae. It can solve the problems of limited percentages and inability to meet industrial scale development, so as to avoid interference and save research and development costs. and research and development time, and the effect of improving breeding efficiency

Active Publication Date: 2013-01-23
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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Problems solved by technology

Although the first two methods can effectively promote the lipid production of algae cells to a certain extent, the percentage of improvement is limited (usually less than 20%), which still cannot meet the requirements of industrial scale development

Method used

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  • Chlorella mutant strain and application thereof

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Embodiment Construction

[0027] The present invention is further described below in conjunction with embodiment.

[0028]The first step, on the aseptic operating table, pick the original wild strain Chlorella (Chlorella kessleri) algae colony that grows well on the solid medium, inoculate into the 50ml Erlenmeyer flask that contains 10ml sterile Kuhl medium, in The temperature is 25 °C, the rotation speed is 150 rpm, and the light intensity is 100 μmol m -2 the s -1 Culture in a shaker with continuous light. Kuhl medium formula: 10g / L glucose, 1g / L potassium nitrate, 89mg / L disodium hydrogen phosphate dodecahydrate, 621mg / L sodium dihydrogen phosphate dihydrate, 246mg / L magnesium sulfate heptahydrate, 9.3mg / L EDTA , 6.9mg / L ferrous sulfate heptahydrate, 14.7mg / L calcium chloride dihydrate, 0.29mg / L zinc sulfate heptahydrate, 0.17mg / L manganese sulfate monohydrate, 0.06mg / L boric acid, 0.002mg / L five Water copper sulfate, 0.012mg / L ammonium molybdate tetrahydrate, add water to a total volume of 100...

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Abstract

The invention relates to a chlorella mutant strain which is bred and used for producing single-cell grease and biodiesel and application thereof. The chlorella mutant strain is collected in CGMCC (China General Microbiological Culture Collection Center) on May 27, 2011, and the collection number is CGMCC No.4917. The chlorella mutant strain is a single-cell grease high-fat mutant strain and can be widely applied to the biodiesel production technology.

Description

technical field [0001] The invention relates to a selected chlorella mutant strain for producing single-cell oil and biodiesel and its application. Background technique [0002] The outstanding environmental protection and renewability of biodiesel has attracted great attention from developed countries, especially resource-poor countries. Biodiesel is mainly obtained from bio fat as raw material, which is converted into fatty acid methyl ester through esterification. The carbon dioxide produced by biodiesel is only 16% to 40% of that of traditional diesel, and the emission of exhaust particles is also reduced by about 30%. At the same time, it can be mixed or used alone without any modification to the existing diesel engine. Changes are needed in the way energy is distributed and in the markets for energy resources that can be used directly as fuel for domestic use and for internal combustion engines. [0003] Bio-fat raw materials for biodiesel mainly come from vegetable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N15/01C12P7/64C12R1/89
CPCY02E50/13C12N15/01C12N1/12C12P7/64C12P7/6463C12R1/89Y02E50/10C12R2001/89C12N1/125
Inventor 王艳秦松
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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