Chlorella mutant strain and application thereof
A technology of mutant strains and chlorella, which is applied in the direction of mutant preparation, single-cell algae, and methods of using algae. It can solve the problems of limited percentages and inability to meet industrial scale development, so as to avoid interference and save research and development costs. and research and development time, and the effect of improving breeding efficiency
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[0027] The present invention is further described below in conjunction with embodiment.
[0028]The first step, on the aseptic operating table, pick the original wild strain Chlorella (Chlorella kessleri) algae colony that grows well on the solid medium, inoculate into the 50ml Erlenmeyer flask that contains 10ml sterile Kuhl medium, in The temperature is 25 °C, the rotation speed is 150 rpm, and the light intensity is 100 μmol m -2 the s -1 Culture in a shaker with continuous light. Kuhl medium formula: 10g / L glucose, 1g / L potassium nitrate, 89mg / L disodium hydrogen phosphate dodecahydrate, 621mg / L sodium dihydrogen phosphate dihydrate, 246mg / L magnesium sulfate heptahydrate, 9.3mg / L EDTA , 6.9mg / L ferrous sulfate heptahydrate, 14.7mg / L calcium chloride dihydrate, 0.29mg / L zinc sulfate heptahydrate, 0.17mg / L manganese sulfate monohydrate, 0.06mg / L boric acid, 0.002mg / L five Water copper sulfate, 0.012mg / L ammonium molybdate tetrahydrate, add water to a total volume of 100...
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