Application method of high-flux plant leaf crusher

A plant leaf and pulverizer technology, which is applied in the field of agricultural scientific research, can solve the problems of unclean mortar cleaning, cross-contamination of samples, time-consuming and labor-intensive, etc., and achieves the effects of convenient sample transfer, easy operation and high efficiency.

Inactive Publication Date: 2013-01-30
王丽华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method of mortar grinding is time-consuming and laborious, and when the number of samples is large, the problem of cross-contamination between samples will occur due to the unclean cleaning of the mortar

Method used

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  • Application method of high-flux plant leaf crusher

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The rapid crushing of embodiment 1. sorghum blade:

[0029] ① Cut the leaves of sorghum except the large veins into small pieces and put them in a shredder.

[0030] ② The amount of blades put in should not exceed 1 / 4 of the volume of the pulverizer.

[0031] ③ Close the shredder cover.

[0032] ④ Put multiple pulverizers into a foam plastic box, inject liquid nitrogen into the box, and the injection volume must submerge all the pulverizers.

[0033] ⑤ Cover the foam box and let it stand for 1 minute.

[0034] ⑥ Use a colander to fish out the pulverizer, and take 36 pieces and put them into one end of a 72-hole centrifuge tube box.

[0035] ⑦ Close the lid of the centrifuge tube box, hold the other end of the centrifuge tube box with both hands, and use the elbow as the fulcrum to vibrate up and down quickly for 10 seconds. The blades in the shredder are smashed to pieces under the strong repeated impact of steel balls.

[0036] ⑧ Open the lid of the box, quickly t...

Embodiment 2

[0038] The rapid crushing of embodiment 2. corn blades:

[0039] ① Remove the large veins from the corn leaves and cut them into small pieces and put them in a shredder.

[0040] ② The amount of blades put in should not exceed 1 / 4 of the volume of the pulverizer.

[0041] ③ Close the lid of the shredder.

[0042] ④ Put multiple pulverizers into a foam plastic box, inject liquid nitrogen into the box, and the injection volume must submerge all the pulverizers.

[0043] ⑤ Cover the foam box and let it stand for 1 minute.

[0044] ⑥ Use a colander to fish out the pulverizer, and take 36 pieces and put them into one end of a 72-hole centrifuge tube box.

[0045] ⑦ Close the lid of the centrifuge tube box, hold the other end of the centrifuge tube box with both hands, and use the elbow as the fulcrum to vibrate up and down quickly for 10 seconds. The blades in the shredder are smashed to pieces under the strong repeated impact of steel balls.

[0046] ⑧ Open the box cover, qui...

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Abstract

A high-flux plant leaf crusher comprises a plastic centrifugal pipe and a steel ball, wherein the steel ball is positioned at the bottom of the crusher. When in use, plant leaves are cut into pieces; the pieces are placed into crushers; the crushers are covered; a plurality of crushers containing samples are added into a foamed plastic box; liquid nitrogen is injected into the box; the foamed plastic box is covered, and are subjected to standing for approximately 1min; the crushers are fetched and are placed in a centrifugal pipe box; and the centrifugal pipe box is covered and is violently oscillated for 10 seconds by using hand, such that the leave samples are completely crushed. The crusher provided by the invention has the advantages of simple operation, high speed, high efficiency, and no cross contamination among the samples.

Description

[0001] Field [0002] The invention belongs to a method for agricultural scientific research, in particular to a method for using a high-throughput plant leaf pulverizer. Background technique [0003] In the process of extracting DNA and protein from plants, the first step is to crush the plant leaves. The existing grinding method is to add the sample and quartz sand (or liquid nitrogen) to the mortar, and grind it with a grinding rod. The sample takes about 1 minute, then the sample is transferred, the mortar is rinsed, wiped clean, and the next sample is ground. This method of mortar grinding is time-consuming and laborious, and when the number of samples is large, the problem of cross-contamination between samples will also occur due to unclean cleaning of the mortar. Therefore, it is necessary to create a convenient and labor-saving, fast and efficient blade shredder and a shredding method that will not cause cross-contamination between samples. Contents of the inventio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B02C23/06B02C17/10
Inventor 王丽华李杰勤刘正余海兵洪阳
Owner 王丽华
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