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Mesenchymal stem cells genetically modified with angiopoietin 1 gene and construction method and application thereof

A technology that promotes angiopoietin and mesenchymal stem cells, applied in the field of biomedicine, can solve problems such as no good, achieve obvious effects, improve lung pathological damage, and have broad application prospects

Inactive Publication Date: 2013-01-30
SHANGHAI PULMONARY HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But how to infuse Ang1 back into the lungs and make it biologically active, there is currently no good method

Method used

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  • Mesenchymal stem cells genetically modified with angiopoietin 1 gene and construction method and application thereof
  • Mesenchymal stem cells genetically modified with angiopoietin 1 gene and construction method and application thereof
  • Mesenchymal stem cells genetically modified with angiopoietin 1 gene and construction method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] 1. Construction of angiopoietin-1 gene-modified mesenchymal stem cells

[0033] 1) Construction of lentiviral vector plasmid expressing Ang1 gene

[0034] 1.1. Extraction of Ang1 gene

[0035] Cloning primers are:

[0036] ANGIF-CTTTTTTGTTAGACAGGATCCGAGTGTGCTGGCAGTACAATGAC AGTTTTCC

[0037] ANGIR-CTCACCATGGTGGCGACCGGTggAAAAATCTAAAGGTCGAATCAT C

[0038] ANG1-seq: TCGTCGTGTCGTGACGTC+ANGIR

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Abstract

The invention discloses mesenchymal stem cells genetically modified with an angiopoietin 1 gene and a construction method and application thereof. The mesenchymal stem cells carry the angiopoietin 1 gene. Tests prove that the mesenchymal stem cells genetically modified with an angiopoietin 1 gene can overexpress angiopoietin 1; after venous re-transfusion, the angiopoietin 1 is overexpressed in the pulmonary tissue, so that pulmonary pathological injuries, pulmonary inflammatory response and pulmonary edema are obviously relieved, and an obvious and long-lasting effect is achieved; and the mesenchymal stem cells genetically modified with angiopoietin 1 gene and the construction method thereof are expected to provide a suitable microenvironment for repair of the pulmonary injuries, and have a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an angiopoietin-1 gene-modified mesenchymal stem cell, a construction method and application thereof. Background technique [0002] Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are common clinical diseases with high morbidity and high mortality. The current global incidence is 8-20 people / 100,000 people / year. Bone marrow-derived mesenchymal stem cells play an important role in the repair of lung injury and inflammation, and are an important potential pathway for lung injury repair. However, research on the repair of lung injury by mesenchymal stem cells has revealed some problems, such as the rare number of exogenous stem cells reaching the lungs, the low level of repair, and the failure to achieve the expected effect. Some researchers used fluorescent protein to trace mesenchymal stem cells (mesenchymal stem cells, MSCs) and pointed out that after ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/867A61K48/00A61P11/00A61L27/38
Inventor 徐金富瞿介明
Owner SHANGHAI PULMONARY HOSPITAL
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