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A kind of lacs gene site related to corn oil content and its application

A corn oil and content technology, applied in the field of corn breeding and molecular biology, can solve the problem that the oil content of high-oil groups is no less than the international level.

Inactive Publication Date: 2016-01-27
HUAZHONG AGRI UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the breeding of high-oil corn in China is relatively late, the oil content of the developed high-oil population is no less than the international level

Method used

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  • A kind of lacs gene site related to corn oil content and its application
  • A kind of lacs gene site related to corn oil content and its application
  • A kind of lacs gene site related to corn oil content and its application

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Embodiment Construction

[0032] The preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings; it should be understood that the preferred embodiments are only for illustrating the present invention, rather than limiting the protection scope of the present invention.

[0033] AM508 population: consists of 473 common corn inbred lines and 35 high-oil corn inbred lines, which have been disclosed in the reference Yang, X.H.etal.Characterizationofaglobalgermplasmcollectionanditspotentialutilizationforanalysisofcomplexquantitativetraitsinmaize.

[0034] Candidate gene resequencing materials: 155 key inbred lines in my country, which have been published in the reference Yang, X.H. et al. Genetic analysis and characterization of a new maize association mapping panel for quantitative trait loci dissection.

[0035] Transcriptome sequencing materials: 368 inbred lines were randomly selected from AM508 inbred lines 15 days after pollination, and...

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Abstract

The invention relates to an LACS (long chain acyl-coenzyme A synthetase) gene locus related to maize oil content and an application thereof, belonging to the field of plant molecular breeding. The locus related to the maize oil content, disclosed by the invention, comprises SNP (single nucleotide polymorphism) positioned at maize reference genome (www.maizesequence.org, 5a.60 edition) chr2.S-149517635, and the mutation of the locus is T / G; and the locus further comprises the locus positioned at InDel-146 / 472 (insertion of 146bp / deletion of 472bp and deletion of 46bp / insertion of 472bp) of the maize LACS gene (GRMZM2G079236) 3'UTR. A molecular marker can be used for high-oil maize breeding under different genetic backgrounds and can be used for maize genetic improvement; and the method and the marker have broad application prospects in the field of maize breeding.

Description

technical field [0001] The invention relates to the application of molecular markers of genes related to corn oil content, and belongs to the fields of corn breeding and molecular biology. Background technique [0002] Corn oil is one of the main products of corn processing. It is a vegetable oil with a variety of antioxidants, high in linoleic acid, and relatively high in vitamin A and vitamin E. The content of linoleic acid and the ratio of linoleic acid to oleic acid determine the quality of vegetable oil, while vitamin A and vitamin E have health care and medicinal value. Edible corn oil is beneficial to human health, so corn oil has the title of "healthy food oil". [0003] Research on high-oil corn in my country began in the 1980s. Professor Song Tongming introduced high-oil corn semi-finished products such as ASKC23, IHOC80, Syn.D.O, and ARYD from abroad, and continued to select and form AIHO, disease-resistant high-oil corn (Syn.D.O.), Lide Gaoyou (RYDHO) and other...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/52C12Q1/68
Inventor 严建兵杨小红李建生李慧
Owner HUAZHONG AGRI UNIV