Method for synthesizing biodiesel with microorganisms in vivo

A technology for synthesizing fatty acids by microorganisms, which is applied in the field of de novo synthesis of biodiesel in microorganisms to achieve the effect of improving synthesis capacity and reducing costs
CN102071228BActive Publication Date: 2013-09-25青岛生物能源与过程研究所
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
青岛生物能源与过程研究所
Publication Date
2013-09-25

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Abstract

The invention relates to a method for synthesizing biodiesel with microorganisms in vivo, which is realized in escherichia coli. The method comprises the following steps of: respectively cloning an acinetobacter wax fat synthetase gene atfA, an escherichia coli acyl coenzyme A synthetase gene fadD, a zymomonas mobilis pyruvate decarboxylase gene pdc and an alcohol dehydrogenase gene adhB gene on a pET expression vector in series to obtain a plasmid pXT11; transferring the plasmid pXT11 into escherichia coli containing a plasmid pMSD8 and a plasmid pMSD15 to obtain an escherichia coli stain XT31; and generating biodiesel by the escherichia coli stain XT31. The method realizes the microbial transformation from sugar to the biodiesel, lays the foundation for biologically preparing the biodiesel from lignocelluloses, and radically solves the problem of insufficient raw material supply in biodiesel preparation. A step of esterifying methanol is not needed in the technology, thereby the cost is greatly reduced and the problems that glycerol as a side product is formed, and the like are avoided.
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Description

technical field

[0001] The invention belongs to the fields of renewable energy and biomass energy, and in particular relates to a method for synthesizing biodiesel from scratch in microorganisms. Background technique

[0002] Biodiesel is mainly prepared from animal and vegetable fats and oils through transesterification process catalyzed by chemical method or enzymatic method. The main bottleneck problems faced by this production method are the shortage of production raw materials, the use of methanol and the removal of glycerin by-products, etc., and the most important problem is the insufficient supply of production raw materials. At present, the main oil resources (such as soybean oil, rapeseed oil, etc.) used in the industry to prepare biodiesel are also necessary for human life, while a large number of oil plants are planted to provide raw materials, and there are insufficient arable land and seasonal climate restrictions. question. Microalgae oil is considered to be...

Claims

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