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Method for detecting fluorine content in water by utilizing escherichia coli

A technology of Escherichia coli and fluorine content, which is applied in the preparation of test samples, color/spectral characteristic measurement, etc., can solve the problems of high cost, high requirements for instrument analysis conditions, and complicated operation, and achieve low cost, easy training, and operation simple effect

Inactive Publication Date: 2015-04-29
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention provides a method for detecting fluorine content in water by using E.

Method used

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  • Method for detecting fluorine content in water by utilizing escherichia coli

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specific Embodiment approach 1

[0015] Specific embodiment one: a kind of method of utilizing Escherichia coli to detect the fluorine content in the water body of this embodiment is carried out according to the following steps:

[0016] 1. Configure LB liquid medium;

[0017] Second, the concentration of the bacterial solution was 10 6 ~10 9 cuf / mL of wild-type E. coli strain liquid and the LB liquid medium in step 1 are mixed at a volume ratio of (1~2):1000, and cultured for 9~11 hours at a temperature of 34~40°C until reaching the platform. period, the plateau bacterial solution was obtained;

[0018] 3. Mix the plateau bacterial liquid obtained in step 2 with the LB liquid medium in step 1 at a volume ratio of 1: (90-110) to obtain a culture bacterial liquid for detecting fluorine content in water;

[0019] 4. Mix the water samples with different fluorine content with the culture solution obtained in step 3 to detect the fluorine content in the water body according to the volume ratio (1~2): 1000, resp...

specific Embodiment approach 2

[0022] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the volume ratio of the wild-type E. coli strain bacterial liquid in step two and the LB liquid culture medium of step one is 1.5:1000, other steps and parameters and specific implementation Method 1 is the same.

specific Embodiment approach 3

[0023] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in Step 2, the culture temperature is 37° C., and the culture time is 10 h. Other steps and parameters are the same as Embodiment 1 or 2.

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Abstract

The invention provides a method for detecting fluorine content in water by utilizing escherichia coli and relates to the method for detecting the fluorine content in water. The method provided by the invention is used for solving the technical problems of the present method for detecting the fluorine content in water that the demand on an instrument analysis condition is high, the operation is complex and the cost is low. The method comprises the following steps: 1) preparing an LB fluid medium; 2) cultivating wild escherichia coli strain liquid to a platform stage; 3) adding the fluorine in different contents into the fresh LB fluid medium containing bacterium fluid; 4) measuring a growing OD (Outside Dimension) value of the bacterium fluid by using an ultraviolet spectrometry photometer; 5) establishing a relation curve and a regression equation between the growing OD value of the bacterium fluid and the fluorine content; and 6) adding a to-be-detected water sample containing the known fluorine content into the fresh LB fluid medium containing the bacterium fluid, and calculating the fluorine content of the to-be-detected water sample according to the regression equation. The method provided by the invention has the advantages of low cost, few steps, convenience in experimental operation and high accuracy and is applied to the field of environmental water quality monitoring.

Description

technical field [0001] The invention relates to a method for detecting fluorine content in water. Background technique [0002] Fluorine is one of the essential trace elements for the human body. Fluoride exists in the form of fluoride ions. Excessive intake of fluorine will endanger health and lead to fluorosis, which is manifested as systemic chronic damage mainly involving teeth and bones. Fluoride pollution refers to the environmental pollution caused by fluorine and its compounds. Fluoride is an accumulative poison. Excessive intake of fluorine will interfere with the activity of enzymes, destroy the metabolic balance of calcium and phosphorus, and cause bone fluorosis with symptoms such as dental plaque and joint deformation. Fluorine is also a protoplasmic poison, which can easily penetrate the cell walls of various tissues and combine with protoplasm, and has the effect of destroying protoplasm. Endemic skeletal fluorosis is the main cause of endemic fluorosis and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/28
Inventor 侯晓丽王云彪张风君
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S