Method for fixing antibody in solid phase carrier in immune adsorption reaction process

Abstract

The invention relates to a method for fixing an antibody in a solid phase carrier in the immune adsorption reaction process. The method is characterized by comprising the following steps: enveloping FITC (fluorescein isothiocyanate) antibody on the solid phase carrier in advance, and then reacting 8-12 hours at 4-6 DEG C; sealing unreacted sites of the solid phase carrier, and then reacting 1-2 hours at 34-37 DEG C; adding an FITC mark antibody, then reacting 1-2 hours at 34-37 DEG C or 8-12 hours at 4-6 DEG C; and finally completing the enveloping of the solid phase carrier. According to the method provided by the invention, the problems that a current enveloped antibody is high in concentration, low in sensitivity, and poor in specificity are solved. The method that an enveloping layer is formed on the solid phase carrier in advance, and then the antibody is fixed has the advantages of simplicity, low cost, strong applicability, wide range and the like.

Description

technical field [0001] The invention relates to a method for immobilizing antibodies on a solid-phase carrier during an immunoadsorption reaction. Background technique [0002] The first condition in the process of immunoadsorption reaction is to immobilize the target antibody on the reaction carrier. The traditional method is that the antibody is immobilized on the carrier by physical adsorption, that is, the antibody is passively adsorbed on the surface of the hydrophobic solid phase carrier by physical force, and then other adsorption sites of the ELISA carrier are blocked by inert proteins. Because the physical adsorption process is relatively simple and random, and the binding sites with the carrier are diversified; and the antibody is blocked under the inert protein, there is a certain steric hindrance in the antigen-antibody binding reaction during the detection process, which affects the immunodetection Reagent sensitivity. And the antibody is distributed unevenly,...

AI Technical Summary

Problems solved by technology

Because the physical adsorption process is relatively simple and random, and the binding sites with the carrier are diversified; and the antibody is blocked under the inert protein, there is a certain steric hindrance in the antigen-antibody binding reaction during the detection process, which affects the immunodetection Reagent sensitivity

Moreover, the antibody is not uniformly distributed, but the site that originally binds to the antigen is bound to the solid-phase carrier, and this part of the antibody loses its function, thereby wasting the antibody, and at the same time occupying the space of the carrier and reducing the sensitivity

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