Unlock instant, AI-driven research and patent intelligence for your innovation.

Novel use of cryptoporus volvatus

A technology of Cryptopora and Cryptopora, which is applied to the application field of Cryptopora in the preparation of porcine reproductive and respiratory syndrome virus inhibitors, can solve problems such as failure, and achieves improving survival rate, reducing titer, reducing high heat effect

Inactive Publication Date: 2013-02-13
CHINA AGRI UNIV
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the prevention and control of PRRS virus infection and transmission mainly rely on vaccines, but they are not successful

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel use of cryptoporus volvatus
  • Novel use of cryptoporus volvatus
  • Novel use of cryptoporus volvatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, prepare porcine reproductive and respiratory syndrome virus inhibitor with Cryptoporus bacterium

[0042] One, the preparation of Cryptoporus extract

[0043] Soak the dry fruiting bodies of Cryptoporia in distilled water, the weight ratio of water to dry fruiting bodies of Cryptoporia is 6:1, soak at 4°C for 2-4 hours, fully mash with a homogenizer to 60 mesh, extract at 4°C for 10 hours , centrifuged at 8000g for 20 minutes, collected the supernatant, and freeze-dried to obtain the Cryptoporus extract. The dry fruiting body of Cryptoporus is obtained by drying fresh fruiting body of Cryptoporus at normal temperature.

[0044] The Cryptopodobacterium extracts used in the experiment of the following step 2 were all dissolved in physiological saline and filtered through a 0.22 micron filter before use.

[0045] 2. Cryptoporus extract inhibits porcine reproductive and respiratory syndrome (PRRS) virus replication in porcine alveolar macrophages and African...

Embodiment 2

[0065] Embodiment 2, prepare porcine reproductive and respiratory syndrome medicine with cryptoporus bacteria

[0066] One, the preparation of Cryptoporus extract

[0067] Soak the dry fruiting bodies of Cryptoporia in distilled water, the weight ratio of water to the dry fruiting bodies of Cryptoporia is 7:1, soak for 2-4 hours at 4°C, fully grind to 80 mesh with a homogenizer, extract for 10 hours at 50°C , centrifuged at 8000g for 10 minutes, collected the supernatant, and freeze-dried to obtain the Cryptoporus extract. The dry fruiting body of Cryptoporus is obtained by drying fresh fruiting body of Cryptoporus at normal temperature. The Cryptoporus extract was dissolved with physiological saline and filtered through a 0.22 micron filter to obtain the Cryptoporus extract injection.

[0068] 2. Cryptoporus extract inhibits the reproduction of PRRS virus in pigs

[0069] 1. Experimental method

[0070] Ten 4-week-old SPF pigs with a body weight of 6-8kg were randomly div...

Embodiment 3

[0080] Embodiment 3, the mechanism of Cryptoporus extract against porcine reproductive and respiratory syndrome virus

[0081] One, the preparation of Cryptoporus extract

[0082] Soak the dry fruiting bodies of Cryptoporia in distilled water, the weight ratio of water to the dry fruiting bodies of Cryptoporia is 6:1, soak at 4°C for 2-4 hours, fully pulverize with a homogenizer to 50 mesh, extract at 4°C for 10 hours , centrifuged at 8000g for 15 minutes, collected the supernatant, and freeze-dried to obtain the Cryptoporus extract. The dry fruiting body of Cryptoporus is obtained by drying fresh fruiting body of Cryptoporus at normal temperature. The Cryptopodobacterium extracts used in the experiment of the following step 2 were all dissolved in physiological saline and filtered through a 0.22 micron filter before use.

[0083] 2. The mechanism of Cryptoporus extract against porcine reproductive and respiratory syndrome virus

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a novel use of cryptoporus volvatus. The novel use of cryptoporus volvatus is at least one of the four uses: 1), an application of cryptoporus volvatus in preparation of an inhibitor for porcine reproductive and respiratory syndrome; 2), an application of cryptoporus volvatus in preparation of a product (such as a drug) for inhibiting copy of a porcine reproductive and respiratory syndrome virus in a host cell; 3), an application of cryptoporus volvatus extract in preparation of an inhibitor for porcine reproductive and respiratory syndrome; 4), an application of cryptoporus volvatus extract in preparation of a product (such as a drug) for inhibiting copy of a porcine reproductive and respiratory syndrome virus in a host cell, wherein the cryptoporus volvatus extract is a water-soluble substance extracted from cryptoporus volvatus sporocarp.

Description

technical field [0001] The invention relates to a new application of cryptoporus, in particular to the application of cryptoporus in preparing porcine reproductive and respiratory syndrome virus inhibitors. Background technique [0002] Porcine reproductive and respiratory syndrome (commonly known as PRRS) (Porcine Reproductive and Respiratory Syndrome, PRRS) is an infectious disease of pigs caused by porcine reproductive and respiratory syndrome virus (PRRS virus, PRRSV). Pigs of all ages are susceptible to the virus. The main symptoms are abortion, stillbirth, mummified fetus and weak fetus after infection of pregnant sows. Infected piglets may suffer from respiratory disorders and high mortality. The disease was first reported in the United States in 1987 and was introduced into my country in the mid-1990s. At present, the disease occurs in almost all pig-raising countries in the world, and has caused great economic losses to the pig industry in my country and the world....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/06A61P31/14
Inventor 王贺祥封文海张薇薇高丽朱孟娟马增强杜芳张哲张瑞刘庆洪
Owner CHINA AGRI UNIV