AaORA gene promoter and application thereof

A promoter and gene technology, applied in the field of AaORA gene promoter, can solve problems such as the undiscovered AaORA gene promoter sequence

Active Publication Date: 2014-06-18
上海阿提密斯生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching the prior art literature, it is found that there is no report related to the AaORA gene promoter sequence of the present invention

Method used

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  • AaORA gene promoter and application thereof
  • AaORA gene promoter and application thereof
  • AaORA gene promoter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] This embodiment relates to the acquisition of the AaORA gene promoter, which specifically includes the following steps:

[0028] Step 1, Cultivate Artemisia annua sterile vaccine

[0029] Artemisia annua seeds were soaked in ethanol with a volume fraction of 75% for 1 min, then soaked in 20% (w / v) NaClO for 20 min, rinsed with sterile water 3 to 4 times, blotted the surface moisture with sterile absorbent paper, and inoculated Cultivate on hormone-free MS solid medium at 25°C under 16h / 8h (light / dark) light, and 14 days later you can obtain sterile A. annua;

[0030] Step 2, Genomic DNA Walking (Genomic DNA Walking) method to clone the genome sequence of the promoter

[0031] In this example, genomic DNA walking was used to isolate the 5' flanking sequence of AaORA gene. For the method of genomic DNA walking, refer to the instructions of Genome WalkerTM Universal Kit (Clontech, 638904). The specific steps are as follows:

[0032] ① Enzyme digestion of genome

[0033]...

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PUM

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Abstract

The invention relates to an AoORA gene promoter, wherein the nucleotide sequence of the promoter is shown in SEQ ID NO.1; and in addition, the invention further relates to application of the AoORA gene promoter in genetic engineering breeding of utilizing plant glandularia tissues to express and produce metabolite. The promoter provided by the invention is capable of inducing genes to be specifically expressed in plant glandularia without bringing damage to the growth and development of plants because the promoter specifically expressed in glandularia carries out genetic operation on the glandularia systems of the plants, thereby being significant in genetic engineering breeding of utilizing the plant glandularia tissues to express and produce metabolite.

Description

technical field [0001] The present invention relates to a gene promoter in the technical field of genetic engineering and its use, in particular to an AaORA gene promoter and its use. Background technique [0002] Artemisia annua L. is an annual herb belonging to the family Asteraceae. Artemisinin is a sesquiterpene lactone compound containing a peroxide bridge structure isolated from its aerial part. It is currently recognized as the most effective drug for treating malaria in the world, especially for cerebral malaria and chloroquine-resistant malaria. efficient. Currently, the most effective treatment for malaria recommended by the World Health Organization is artemisinin combination therapy (ACTs). At present, the main source of artemisinin is extracted from the aerial parts of Artemisia annua plants. However, the content of artemisinin in Artemisia annua is very low (0.01%-1%), which limits the large-scale commercial production of this drug. [0003] AaORA is an AP2-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A01H5/00
Inventor 唐克轩陆续张芳源沈乾张凌江伟民吕宗友
Owner 上海阿提密斯生物科技发展有限公司
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