Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biological sample pretreatment method and apparatus

A technology of organisms and reagents, applied in the direction of measuring devices, biological material analysis, and preparation of test samples, which can solve complex processing and other problems

Active Publication Date: 2013-02-20
HITACHI HIGH-TECH CORP
View PDF6 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] Furthermore, the reaction of the sample requires complicated processing as described above, and requires work and time.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biological sample pretreatment method and apparatus
  • Biological sample pretreatment method and apparatus
  • Biological sample pretreatment method and apparatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] figure 1 It is a figure explaining the preprocessing flow of the sample preprocessing method in Example 1 of this invention. figure 1 The pretreatment flow 103 is the pretreatment flow in Embodiment 1 of the present invention, and the whole blood treatment and the solid phase extraction treatment are processed together.

[0045] figure 1 The flow of whole blood processing (processing flow 101 ) and solid-phase extraction processing (processing flow 102 ) is shown separately for comparison with the pretreatment of the present invention.

[0046] (Sample preparation procedure and solubility of immunosuppressants)

[0047] In the processing flow 101, the whole blood sample is dispensed into the filter medium, and the hemolysis treatment solution (zinc sulfate aqueous solution, etc.) is dispensed, followed by stirring. Then, the protein-removing liquid (such as methanol) is dispensed and stirred, and then filtered (or centrifuged).

[0048] In this case, for example, in...

Embodiment 2

[0064] Next, a biological sample preprocessing device as a second embodiment of the present invention will be described. This embodiment 2 becomes the apparatus constitution of suspension mode.

[0065] image 3 It is an explanatory diagram of a sample pretreatment device of a suspension system as Example 2 of the present invention.

[0066] Such as image 3 As shown in (C), the sample pretreatment device 301 is characterized in that it is integrated by inserting the filter device 302 into the solid phase extraction device 303 .

[0067] This sample pretreatment device 301 is characterized in that a filter device 302 is arranged on the upstream side, and a solid phase extraction device 303 is arranged on the downstream side, and the filtrate from the filter device 302 is directly supplied to the solid phase without temporarily recovering it. Tool 303 was extracted for testing.

[0068] The filter device 302 is composed of a filter reservoir 304 and a filter unit 305 . In ...

Embodiment 3

[0090] Next, a sample pretreatment device as a third embodiment of the present invention will be described. This Example 2 has a syringe type (non-detachable) equipment configuration.

[0091] Figure 4 It is a diagram showing the configuration of a syringe-type (non-detachable) sample preprocessing device (sample preprocessing device) 401 as a third embodiment of the present invention.

[0092] exist Figure 4 In this case, the sample preprocessing device 401 has a structure that cannot be attached and detached so that the filter device 402 and the solid phase extraction device 403 cannot be separated from each other.

[0093] As for the sample pretreatment device 401, a filter device 402 is arranged on the upstream side, and a solid phase extraction device 403 is arranged on the downstream side, and the filtrate from the filter device 402 is directly supplied to the solid phase extraction device without temporarily recovering it. 403 for testing.

[0094] The filter devi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Disclosed is a sample treatment apparatus which can subject a filtrate obtained after a reaction treatment to a solid phase extraction treatment without the need of carrying out a primary collection of the filtrate. A necessary amount of a solid phase extracting agent (308) is added to a solid phase extraction instrument (303) to carry out conditioning. An aqueous solution is added to the solid phase extracting agent (308), and a supernatant is discarded. A filter instrument (302) is attached to an opening of the solid phase extraction instrument (303), and a sample is added to a filter reservoir (304). A hemolyzing agent is added to the sample, and the resulting mixture is fully agitated to cause the disruption of blood cells. A protein-denaturing solution is added to the mixture, and the resulting mixture is fully agitated to denature a protein contained in the sample, thereby forming an aggregate. The solution is centrifuged to leave the aggregate on the filter instrument (302) side, and the filtrate is supplied to the solid phase extracting agent through a filter unit (305) without collecting the filtrate. A necessary amount of a washing solution is added to the solid phase extraction instrument (303), the resulting mixture is agitated and centrifuged, and a supernatant is discarded. A necessary amount of an eluting solution is added to the solid phase extraction instrument (303), the resulting mixture is fully agitated and centrifuged, and a supernatant is discarded.

Description

technical field [0001] The present invention relates to a biological sample pretreatment method and device for performing pretreatment for hemolysis, deproteinization, and solid phase extraction of a sample. Background technique [0002] For example, in the extraction of a target component from a multi-component blood sample, there are various techniques that use physical properties such as size, weight, and shape, and biochemical properties such as solubility and affinity of the target component as indicators. . Especially with regard to the extraction of trace components in biological samples such as blood, separation techniques using chromatography are being developed, and fillers suitable for target components are being developed and methods are being optimized. Even in the case of using such a high-level separation technology, in order to efficiently extract target trace components from blood composed of various cells and multi-components showing different properties, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/28
CPCG01N1/34G01N1/28G01N1/405G01N2035/1053Y10T436/255
Inventor 神田胜弘野上真伊藤伸也和气泉
Owner HITACHI HIGH-TECH CORP
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com