Unlock instant, AI-driven research and patent intelligence for your innovation.

An ELISA kit for detecting the activity of the trkb receptor pan-tyr site and its application method

A kit and site technology, applied in the field of medical biology

Active Publication Date: 2014-10-22
武汉远征世纪制药有限公司
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ELISA products of Cell Signaling cannot meet this need.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An ELISA kit for detecting the activity of the trkb receptor pan-tyr site and its application method
  • An ELISA kit for detecting the activity of the trkb receptor pan-tyr site and its application method
  • An ELISA kit for detecting the activity of the trkb receptor pan-tyr site and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] Cells, Reagents, Mice and Rats

[0028] BDNF was purchased from Peprotech (Catalog #450-02). Anti-817 phosphorylated TrkB antibody was purchased from Epitomics (Product No. #2149-1). Anti-TrkB antibody was purchased from Cell signaling (Product No. #4603S). Anti-Trk C14 antibody was purchased from Santa Cruz (Catalog #SC-11). Anti-473 phosphorylated Akt, anti-Akt, and anti-phosphorylated Erk1 / 2 antibodies were purchased from Cell Signaling. Wild-type C57BL / 6 mice were kept in a pathogen-free environment according to the requirements of Emory School of Medicine. Timed pregnant CD rats were purchased from Charles River. All reagents not mentioned above were purchased from Sigma. 7,8-Dihydroxyflavone was purchased from TCI Company. Biotinylated 7,8DHF and biotinylated NAS were synthesized by the laboratory of Professor Mark Goodman at Emory University. Rat cortical neurons were derived from E17 or E18 rat embryos.

[0029] Primary rat cortical neuron culture

[00...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an ELISA kit for detecting TfkB acceptor pan-Tyr site activation level. The kit can carry out quantitative determination on the activation level of an endogenous TrkB acceptor in from E17embryo after the endogenous TrkB acceptor is stimulated by its constant parent ligand BDNF. BDNF can induce the phosphorylation of a plurality of tyrosine residues in the intracellular segment of the TrkB acceptor, and the induction has time dependence and dosage dependence. The process can be detected by using the TrkC-14 antibody and phosphorylated tyrosine antibody (antiPY99 general antibody ) after the TrkB acceptor is fixed on a 96 orifice plate. Accordingly, the invention provides a strong tool for the quantitative determination of the activation level of the endogenous TrkB acceptor in primary neuron culture and brain tissues.

Description

technical field [0001] The invention belongs to the field of medical biotechnology, and relates to an ELISA kit for detecting the activation level of a TrkB receptor pan-Tyr site and a use method thereof. Background technique [0002] Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophic factor family, which includes nerve growth factor (NGF), NT-3, NT-4 / 5 (Thoenen, 1991). BDNF, like other neurotrophic factors, has the biological activity of affecting neuronal cells through the TrkB receptor tyrosine kinase pathway (Huang et al., 2008; Kaplan and Miller, 2000). TrkB is one of the most widely distributed neurotrophic factors in the brain, and is highly enriched in the cerebral cortex, hippocampus, striatum, and brainstem (Shelton et al., 1995). Binding of BDNF to TrkB triggers the latter's dimerization through a change in compliance and autophosphorylation of tyrosine residues, resulting in mitogen-activated protein kinase (MAPK), including mitogen-activat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N21/31
Inventor 叶克强
Owner 武汉远征世纪制药有限公司