In-vitro efficient seedling cultivation method synchronous in rooting and growing by utilizing tender stem segments of thuja koraiensis

Abstract

The invention relates to plant propagation and in particular relates to an in-vitro efficient seedling cultivation method synchronous in rooting and growing by utilizing tender stem segments of thuja koraiensis. A culture medium for the simultaneous growing of roots and the stem segments comprises the components: the culture medium+ 0.06-0.08mg.L-1 of ZT (Zeatin)+ 0.01-0.03mg.L-1 of NAA (NaphthylAcetic Acid)+ 0.02-0.04mg.L-1 of IAA (Indole Acetic Acid); in the culture medium, 8.0g.L-1 of agar and 15g.L-1 of cane sugar are added; and the method comprises the steps of: adjusting the pH (potential of Hydrogen) value of the culture medium to be at 5.6, culturing the tender stem segments under the conditions that the photoperiod is 10h.d-1, the illumination intensity is 900lx and the temperature is 23-27 DEG C, planting small plants after the expanding propagation of the seedlings in a matrix comprising the components of disinfected peat, rotten pine needle and river sand in the ratio of 4:3:1, preserving the moisture and the temperature through film mulching, controlling the temperature in the range of 16-20 DEG C and adopting the 10h.d-1 of natural lighting. By adopting the method, the survival rate of the small plants can be up to more than 95.0%, and therefore, the needs of industrial seedling cultivation can be satisfied.

Description

[0001] technical field [0002] The invention relates to a kind of plant propagation, that is, a high-efficiency seedling raising method using the rooting and growth synchronization of the young stem section of Thuja japonica in test tube. Background technique [0003] In the prior art, Korean Thuja ( Thuja koraiensis Nakai) alias Changbai arborvitae, Cupressaceae Thuja is evergreen small trees or shrubs. It has economic, medicinal and ornamental value. It is a second-class national key protected plant in my country. Only in the Hongtu Mountain area of ​​Changbai Mountain National Nature Reserve, there is a large population area, and there are only sporadic distributions in other areas. It is designated as a endangered species by the "List of Rare and Endangered Plants in China (Volume 1)", and as a second-class national key protected plant in the "List of Rare and Endangered Plants in China (First Batch)". It is listed as Endangered Species (EN) by the "Red List of Speci...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a solution for the lack of wild resources of Korean Thuja cypress, the large consumption of wild resources by conventional methods such as cuttings and seeds, the difficulty of rooting and germination, the low survival rate of transplanting, and the serious shortage of wild resources such as felling and digging. An efficient one-step seedling raising method with feasible technology and high rooting rate using the young stems of Thuja japonica in test tubes to root and grow synchronously

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