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Dynamic high-pressure microfluidization-enzymolysis method for preparing hypoallergenic beta-lactoglobulin

A high-pressure micro-jet and lactoglobulin technology, which is applied in the field of dairy processing and the production of hypoallergenic β-lactoglobulin, can solve the problems of no β-lactoglobulin products and less statistical reports on milk allergy , to achieve the effect of eliminating allergies, reducing allergies, reducing or allergies

Inactive Publication Date: 2013-03-13
NANCHANG UNIV
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Problems solved by technology

In China, the problem of milk allergy has not attracted enough attention, and there are few statistical reports on milk allergy, especially for β-Lg allergy
[0006] After searching, there is no report on the production of low-sensitivity β-lactoglobulin products using dynamic high-pressure microfluidic technology combined with enzyme treatment.

Method used

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  • Dynamic high-pressure microfluidization-enzymolysis method for preparing hypoallergenic beta-lactoglobulin

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Embodiment Construction

[0017] Below in conjunction with embodiment the present invention is described in further detail:

[0018] Weigh 1g of β-lactoglobulin, dissolve it in 1L of secondary deionized water at room temperature, and use a magnetic stirrer to stir at a speed of 50-80rpm for 5-10min to completely dissolve the protein; use a dynamic high-pressure microjet at a pressure of 160MPa The β-lactoglobulin solution was treated 3 times, and the sample was collected for lyophilization; 118.8 mg of the lyophilized β-lactoglobulin sample was dissolved in 10 mL of 50 mM, pH 7.8 and ionic strength of 0.08 M (NaCl) Prepare in Tris buffer; weigh 0.066mg trypsin (10,000U / mg) and dissolve in 5mL Tris buffer of 50mM, pH7.8 and ionic strength 0.08M (NaCl) for use; weigh trypsin inhibitor 110.6 mg was dissolved in 2 mL of 50 mM Tris buffer with pH 7.8 and ionic strength of 0.08 M (NaCl) for use; according to the enzyme-to-substrate ratio of 1:40, 250 μL of trypsin solution was added to 10 mL of β-lactate In...

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Abstract

A dynamic high-pressure microfluidization-enzymolysis method for preparing hypoallergenic beta-lactoglobulin. Beta-lactoglobulin in milk is used as a main raw material, and a novel physical processing means of dynamic high-pressure microfluidization technology is combined with trypsin enzymolysis technology to treat the beta-lactoglobulin, so as to obtain the hypoallergenic beta- milk globulin product. The hypoallergenic beta-lactoglobulin prepared by the method has good functionality; and the method can effectively reduce or eliminate anaphylaxis of beta-lactoglobulin. The invention is highly safe and without side effect.

Description

technical field [0001] The invention relates to a processing method for producing hypoallergenic β-lactoglobulin, which belongs to the research field of dairy processing technology. Background technique [0002] Milk and its products are one of the important sources of human food protein and are of great significance to human health. However, recent studies have found that the protein in milk can cause allergic reactions and seriously threaten human health, and has become one of the hot spots in food safety research. In China, the problem of milk allergy has not attracted enough attention, and there are few statistical reports on milk allergy, especially β-Lg allergy. In our country, the methods commonly used to treat milk allergy in infants and young children are: stop using milk products, switch to milk substitutes, and take specific drugs. But these treatment methods are all only temporary, can not fundamentally solve the problem of infant allergy, and also easily cause...

Claims

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Application Information

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IPC IPC(8): A23J3/34A23J3/08
Inventor 钟俊桢刘成梅刘伟
Owner NANCHANG UNIV
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