Method for preparing porcine pseudorabies live vaccine by using passage CEF (chicken embryo fibroblast)

Abstract

The invention discloses a method for preparing a porcine pseudorabies live vaccine by using the passage CEF (chicken embryo fibroblast), comprising the following steps: firstly, inoculating the primary CEF in the porcine PRV (pseudorabies virus) low virulent strain to obtain a low porcine PRV; then, preparing a DF-1 cell monolayer; next, inoculating the DF-1 cell monolayer in the prepared low porcine PRV; subsequently, putting the DF-1 cell monolayer in a constant-temperature incubator to incubate; and finally, observing the cytopathy, harvesting a virus solution at the right time, freezing and drying the harvested virus solution in vacuum to obtain the porcine pseudorabies live vaccine. According to the method, the passage CEF (DF-1) replaces the primary CEF to culture the PRV, and the process for culturing is optimized systematically, the PRV is easier to culture, the obtained PRV has higher viral titer, the vaccine has more stable quality, and the production cost is lower.

Description

technical field [0001] The invention belongs to the technical field of cultivation of veterinary biological products, in particular to a method for preparing live porcine pseudorabies vaccines by using subcultured chicken embryo fibroblasts. Background technique [0002] Pseudorabies, also known as Aujeszky's disease, is a kind of a variety of livestock, poultry and wild animals caused by pseudorabies virus (Pseudora-bies virus, PRV). Acute infectious disease with inflammation as the main symptom. The virus belongs to the Herpesviridae (Herpesviridae) subfamily of herpesvirus A (Alphaherpesvirinae of alphavirinae), so it is also known as Suid herpesvirus ?, infectious bulbar paralysis virus or scrapie virus. The infection spectrum of this virus is extremely wide, so that 40 kinds of animals can be infected, and pigs are the most important storage host and carrier of this virus, thus playing an important role in the spread of this virus. Due to the great variety of infected...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to propose a method for preparing porcine pseudorabies live vaccine with subcultured chicken embryo fibroblasts in view of the deficiencies in the prior art. The method uses passaged chicken embryo fibroblasts (DF-1) to replace primary chicken Embryonic fibroblast CEF cultures attenuated PRV, and systematically optimizes the culture process to overcome the defects of complexity, high cost, and unstable vaccine quality