Method for researching chemical components of blueberry fruit
A blueberry, chemical technology, applied in the direction of scientific instruments, material separation, analysis of materials, etc.
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Embodiment 1
[0032] Grind the fresh patriot blueberry fruit with a juicer, accurately weigh 5g of the puree, immerse the puree in 100mL of methanol solution containing 0.1% formic acid, extract at room temperature for 2 hours, centrifuge at 1500rpm for 10 minutes, and take the supernatant; Put 1 mL of the supernatant in a centrifuge tube, dry it with a nitrogen blower, add 0.5 mL of BSTFA, put the centrifuge tube in an oven, and keep it at 80°C for 1 hour to obtain a derivatization solution; use a GCMS instrument to test the derivatization solution to obtain For the total ion chromatogram of the derivatization solution, see figure 2 A total of 40 compound structures were identified through spectrum library search and manual spectrum analysis, including 12 acid compounds, 6 alcohol compounds, 4 phenolic compounds, 7 ester compounds, and 8 small molecular sugar compounds , 1 ketone compound, and 2 alkane compounds.
Embodiment 2
[0034] Grind the fresh fruit of Patriot blueberry with a juicer, accurately weigh 5g of the puree, immerse the puree in 150mL of 30% methanol solution, extract at room temperature for 2 hours, centrifuge at 1500rpm for 10 minutes, and take the supernatant; accurately measure the supernatant Put 1 mL in a centrifuge tube, dry it with a nitrogen blower, add 0.5 mL of BSTFA, put the centrifuge tube in an oven, and incubate at 100°C for 1 hour to obtain a derivatization solution; use a GCMS instrument to test the derivatization solution to obtain the total derivatization solution Ion current diagram, see image 3 A total of 34 compound structures were identified through spectral library search and manual spectral analysis, including 12 acid compounds, 6 alcohol compounds, 1 phenolic compound, 6 ester compounds, 1 phenolic compound, small There are 4 molecular sugar compounds, 2 ketone compounds, and 2 other compounds.
Embodiment 3
[0036] Grind the fresh fruit of Patriot Blueberry with a juicer, accurately weigh 5g of the puree, immerse the puree in 100mL of 50% acetone solution, extract at room temperature for 2 hours, centrifuge at 1500rpm for 10 minutes, and take the supernatant; accurately measure the supernatant Put 1mL in a centrifuge tube, dry it with a nitrogen blower, add 1mL of BSTFA, put the centrifuge tube in an oven, and keep it warm at 100°C for 1 hour to obtain a derivatization solution; use a GCMS instrument to test the derivatization solution to obtain the total ions of the derivatization solution flow diagram, see Figure 4 A total of 25 compound structures were identified through spectral library search and manual spectral analysis, including 8 acid compounds, 1 phenolic compound, 3 ester compounds, 7 small molecule sugar compounds, and 3 ketone compounds , 3 other compounds.
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