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Preparation method for triploid grouper

A grouper and triploid technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of long breeding work, unpredictable marine ecological environment, and easy escape of hybrid diploids. To achieve the effect of simple operation, fast growth and huge economic value

Active Publication Date: 2013-04-03
SUN YAT SEN UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] At present, the traditional grouper breeding method has a long breeding period and slow progress in breeding work, and the hybrid diploid in the grouper hybrid breeding model is prone to escape, which will cause unpredictable and serious consequences to the marine ecological environment

Method used

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  • Preparation method for triploid grouper
  • Preparation method for triploid grouper
  • Preparation method for triploid grouper

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Embodiment 1

[0020] The mitochondrial cytochrome b (Cyt b) genes of five species of grouper in the coastal waters of China were amplified by PCR, and the genetic distance was calculated by using the MEGA3.1 software and the Kimura double-parameter model. The specific species of grouper are as follows: red-spotted grouper (E.akaara), brown-spotted grouper (E.fuscoguttatus), saddle-spotted grouper (E.lanceolatus), oblique-spotted grouper (E.coioides) and P. leopardus (P. leopardus). Select healthy grouper as hybrid broodstock.

[0021] Table 1 The primer sequences of grouper mitochondrial cytochrome b (Cyt b) gene amplified by PCR are as follows

[0022]

[0023] Table 2 Genetic distances among thirteen species of grouper

[0024]

[0025]

[0026] 1-2 months before the breeding season, select healthy female oblique-banded grouper and male saddle-banded grouper as brood fish with bright color, no damage to the body surface, bright red gill filaments and no parasites, and the genet...

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Abstract

The invention discloses a preparation method for a triploid grouper, which comprises the following steps: selecting different types of triploid groupers with the genetic relationship of 0.140 to 0.145 genetic distances as parent fishes; adopting a dry method to perform artificial fertilization and distant hybridization; transferring germ cells into seawater after fertilization to perform cold shock treatment; and catching and domesticating for culture of seedlings and identifying the triploid groupers. The preparation method has the advantages of short period for breeding, fast growth speed and no influence on the marine ecological environment.

Description

technical field [0001] The invention belongs to the field of fish genetics and breeding, and in particular relates to a preparation method of triploid grouper. Background technique [0002] Grouper belongs to Perciformes (Perciformes), Serranidae (Serranidae), grouper subfamily (Epinephelinae), is a famous and precious fish in the world. Such as edible fish, deeply like by consumers in general. Grouper has become the main object of mariculture in Guangdong, Hainan, Fujian, Guangxi and other places along the southern coast of China, with huge economic value. [0003] A triploid is an individual with three sets of chromosomes, whereas a normal individual has only two sets of chromosomes. The germinal cells of triploid individuals cannot normally synapse during meiosis and cannot produce normal gametes. During the development of triploid individuals, the abnormal development of gonads saves reproductive energy and uses this part of energy for In addition, triploid individual...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 张勇黄文张海发王韦民李水生王燕肖玲刘晓春林浩然
Owner SUN YAT SEN UNIV
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