Method for preparing aureusidin in liverwort by superhigh pressure technique

An ultra-high pressure technology, the technology of snapdragon, is applied in the field of purification of snapdragon and snapdragon in the enrichment and purification of Diqian by using ultra-high pressure technology and macroporous adsorption resin, which can solve the problem of low extraction purity and low extraction rate, etc. problems, to achieve the effect of high extraction purity, high extraction rate, and stable physical and chemical properties

Inactive Publication Date: 2013-04-10
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the disadvantages of relatively low extraction rate and low extraction purity of conventional techniques, and provide a method for enriching and purifying aureus in Radix Diana using ultra-high pressure technology and macroporous adsorption resin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: A method for enriching and purifying aureusidin in Radix chinensis using ultra-high pressure technology and macroporous adsorption resin, the steps are as follows:

[0023] (1) After crushing 500g of dry ground money, pass it through a 40-mesh sieve, add 10 times the volume of ethanol with a concentration of 80%, soak it for 1 hour, put it into a pressure-resistant bag, and process it at 20°C with an ultra-high pressure of 200MPA, After 4 minutes, the ethanol was recovered and vacuum-dried at 60°C to obtain the crude extract of aureusidin;

[0024] (2) Adsorbing the obtained aureusidin crude extract with a macroporous adsorption resin, the macroporous resin used is the D4006 type resin produced by Tianjin Nankai University, and the adsorption is static adsorption or dynamic adsorption;

[0025] (3) Elute the macroporous resin with 30%, 40%, 50%, 60%, 70%, 80%, and 90% ethanol at different concentrations, track and detect with thin-layer chromatography, and co...

Embodiment example 2

[0031] Implementation case 2: A method for enriching and purifying aureusidin in Radix chinensis by using ultra-high pressure technology and macroporous adsorption resin, the steps are as follows:

[0032] (1) After crushing 500g of dry ground money, pass through a 50-mesh sieve, add 15 times the volume of ethanol with a concentration of 85%, soak it for 1 hour, put it in a pressure-resistant bag, and process it at 20°C with an ultra-high pressure of 300MPA. After 6 minutes, the ethanol was recovered and vacuum-dried at 60°C to obtain the crude extract of aureusidin;

[0033] (2) Adsorbing the obtained aureusidin crude extract with a macroporous adsorption resin, the macroporous resin used is the NKA-9 type resin produced by Tianjin Nankai University, and the adsorption is static adsorption or dynamic adsorption;

[0034] (3) Elute the macroporous resin with 30%, 40%, 50%, 60%, 70%, 80%, and 90% ethanol at different concentrations, track and detect with thin-layer chromatograp...

Embodiment example 3

[0040] Implementation case 3: A method for enriching and purifying aureusidin in Radix chinensis by using ultra-high pressure technology and macroporous adsorption resin, the steps are as follows:

[0041] (1) After crushing 500g of dry ground money, pass it through an 80-mesh sieve, add 20 times the volume of ethanol with a concentration of 90%, soak it for 1 hour, put it in a pressure-resistant bag, and process it at 20°C with an ultra-high pressure of 400MPA. After 8 minutes, the ethanol was recovered, and dried in vacuum at 70°C to obtain the crude aureusidin extract;

[0042] (2) Adsorbing the obtained aureusidin crude extract with a macroporous adsorption resin, the macroporous resin used is the AB-8H type resin produced by Tianjin Nankai University, and the adsorption is static adsorption or dynamic adsorption;

[0043](3) Elute the macroporous resin with 30%, 40%, 50%, 60%, 70%, 80%, and 90% ethanol at different concentrations, track and detect with thin-layer chromato...

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PUM

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Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for purifying aureusidin in liverwort by a superhigh pressure technique and macroporous resin enrichment. The method has characteristics that the extraction speed of aureusidin by the superhigh pressure technique is high; the time is saved; the extraction rate is high; aureusidin is separated and purified by a macroporous adsorption technique; the adsorptive selectivity to aureusidin is good; the adsorption is rapid; the resolution is rapid; the adsorption capacity is larger; the separation effect is obvious; the extraction purity is high; semifinished aureusidin with the content above 30% and finished aureusidin with the content above 90% can be obtained; and the defects that the conventional extraction rate is lower and the extraction purity is low are overcome.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for purifying aureusidin, in particular to a method for enriching and purifying aureusidin in Radix chinensis by using ultra-high pressure technology and macroporous adsorption resin. Background technique: [0002] It is one of the liverworts named by Linnaeus in 1753, and it is widely distributed all over the world. Alias, baron bone, pus scab grass, rice seaweed, lichen; the plant body is flat, band-like, with multiple dichotomous branches, light green or dark green, about 1 cm wide, up to 10 cm long, with edges Slightly wavy, mostly intertwined and grown in sheets. There is a hexagonal air chamber on the back, and the air hole is chimney-shaped, and there are many upright vegetative filaments inside. The basic tissue of the thallus is 12-20 layers of cells thick; there are 6 rows of purple scales on the ventral surface, and there are heart-shaped attachments ...

Claims

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Application Information

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IPC IPC(8): C07D307/83
Inventor 万莉姚德坤张亚红姚德利鲁桂凤
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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