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Zoobenthos in-situ culture method for nutrition and excretion study

A benthic and in situ culture technology, applied in the field of benthic animal culture and in situ culture of benthic animal nutrient excretion, can solve the problems of environmental sensitivity and few researchers, improve the accuracy of measurement and ingenious design , suitable for large-scale promotion and application

Inactive Publication Date: 2014-08-20
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Benthic animals are an important part of the bottom ecosystem of a water body, and they perform various functions in the food web of the aquatic ecosystem. What role does it play in material circulation and energy flow, there are very few researchers

Method used

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  • Zoobenthos in-situ culture method for nutrition and excretion study
  • Zoobenthos in-situ culture method for nutrition and excretion study

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Embodiment Construction

[0021] In order to understand the technical content of the present invention more clearly, the following examples are given in detail.

[0022] 1. Experimental materials:

[0023] Equipment and materials: suction filter, muffle furnace, spectrophotometer, A / E Glass Membrane Filter (1μm, 47㎜) from Pall Corporation, Nalgene jar (plastic jar, Tiangen Biochemical Technology Co., Ltd., 125ml), etc. ;

[0024] Reagents: Nessler's reagent, sodium potassium tartrate solution, ascorbic acid solution, molybdate solution, hydrochloric acid, etc.

[0025] 2. Experimental steps:

[0026] 1 Put the A / E glass fiber filter membrane (1μm, 47mm) in a muffle furnace and bake at 450°C for 3 hours, and then perform acid treatment to remove organic carbon.

[0027] 2. Number and weigh the cleaned centrifuge tubes, and place them here after the experiment on benthic animals (not molluscs) for nutrient excretion. Molluscs are placed in corresponding ziplock bags.

[0028] 3 Acid treatment of 125...

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Abstract

The invention relates to a zoobenthos in-situ culture method for nutrition and excretion study. The method includes the steps of firstly, collecting surface lake water at a sampling spot, and filtering to remove particles with granule size smaller than 1micrometer; secondly, collecting bottom sediment at the sampling spot, and picking out zoobenthos; thirdly, placing the zoobenthos in the surface lake water which is filtered in the step 1, and placing the zoobenthos at the sampling spot underwater for in-situ culture; culturing for 2h if underwater temperature is not smaller than 15 DEG C; and culturing for 4h if the underwater temperature is not larger than 15 DEG C. preferentially, in the step 1, the surface lake water is leached by 1-micrometer A / E glass fiber filter membranes; in the step 3, the surface lake water is held in a culture bottle which is subjected to acid pretreatment. The zoobenthos in-situ culture method for nutrition and excretion study is ingenious. The zoobenthos are subjected to in-situ culture, migration is reduced as far as possible, habitats of the zoobenthos are directly simulated in the open air, excretion rate of the zoobenthos in water can be reflected truly, and a reasonable system scheme for study on nutrient and excretion mechanism in zoobenthos is provided. The zoobenthos in-situ culture method for nutrition and excretion study is applicable to large-scale popularization and application.

Description

technical field [0001] The invention relates to the technical field of animal culture methods, in particular to the technical field of benthic animal culture methods, and specifically refers to an in-situ culture method for benthic animal nutrition and excretion. Background technique [0002] Benthic animals are an important part of the bottom ecosystem of a water body, and they perform various functions in the food web of the aquatic ecosystem. What role does it play in material circulation and energy flow, there are very few researchers. [0003] Therefore, it is necessary to provide a culture method for benthic nutrient excretion, which can truly reflect the excretion rate of benthic animals in water bodies, and provide a reasonable system solution for studying the nutrient excretion mechanism of benthic animals. Contents of the invention [0004] The purpose of the present invention is to overcome the above-mentioned shortcoming in the prior art, provide a kind of ben...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 胡忠军王思卿张颖张新磊凡迎春黄翠
Owner SHANGHAI OCEAN UNIV