Protein sequence with enterocyte protective function and application thereof

A technology of intestinal epithelial cells and protein sequences, which can be used in medical preparations containing active ingredients, peptide/protein components, digestive system, etc.

Inactive Publication Date: 2013-04-17
刘志华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, with the extensive use of antibiotics, hormones, and chemical drugs, coupled with the influence of various toxic and harmful substances in food, the microbial flora in the human body has been severely damaged, and various interdependent microbial flora have lost The original balance is lost, resulting in a decline in the immunity of the immune system of the human body

Method used

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  • Protein sequence with enterocyte protective function and application thereof
  • Protein sequence with enterocyte protective function and application thereof
  • Protein sequence with enterocyte protective function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1, inhibiting the adhesion of EIEC / EPEC to NCM460 cells

[0020] For experimental methods, please refer to the literature: Liu Z, Shen T, Zhang P, Ma Y, Qin H, Lactobacillus plantarum surface layer adhesive protein protects intestinal epithelial cells against tight junction injury induced by enteropathogenic Escherichia coli, Mol Biol Rep , 2011; 38(5): 3471-3480

[0021] As shown in Figure 1, the adhesion rate of EIEC to NCM460 cells was 15%-28%, and the adhesion rate of EPEC to NCM460 cells was 20%-45%. However, when the protein sequence (I58) of the present invention was pretreated (I58+EIEC, I58+EPEC), the adhesion rates of EIEC and EPEC were significantly reduced, both falling below 10%. Compared with pretreatment with Lactobacillus plantarum (LP+ EIEC, LP+EPEC) are similar, or even better.

[0022] However, after adding anti-MIMP (anti-I158) to block its effect, the protective effect of the protein sequence of the present invention disappeared (P<0.05...

Embodiment 2

[0023] Example 2, Inhibition of EIEC / EPEC-induced decrease in NCM460 cell permeability (TER)

[0024] Test method reference: Qin H, Zhang Z, Hang X, Jiang Y. L., Plantarum prevents enteroinvasive Escherichia coli-induced tight junction proteins changes in intestinal epithelial cells, BMC Microbiol , 2009; 9: 63

[0025] The experimental results shown in Figure 2 showed that after EIEC / EPEC intervention, the TER value of NCM460 cells was significantly decreased compared with the control group (P Figure 2A , Figure 2B ), but after I58 pretreatment, the TER value of NCM460 cells increased significantly. After 24 hours, the permeability of EIEC to NCM460 cells increased from about 25% to about 65-70%, and the permeability of EPEC to NCM460 cells increased from 30 % rises to about 70~75%, which is similar to the effect of direct use of Lactobacillus plantarum (LP).

[0026] However, after adding anti-MIMP (anti-I158) to block its effect, the protective effect of the protein s...

Embodiment 3

[0027] Example 3, reducing the destructive effect of EIEC / EPEC on tight junction protein (TJ) and F-actin

[0028] Test method reference: Qin H, Zhang Z, Hang X, Jiang Y. L., plantarum prevents enteroinvasive Escherichia coli-induced tight junction proteins changes in intestinal epithelial cells, BMC Microbiol , 2009; 9: 63

[0029] As shown in Figure 3, fluorescence staining showed that the expression of TJ-related proteins in the control group showed that the fluorescence was distributed along the cell membrane with clear boundaries and a network structure. After EIEC / EPEC infected Caco-2 cells, the fluorescence distribution among the cells was obviously scattered and the intensity was weakened. The gap between cells increases, and the fluorescence intensity of TJ-related proteins is significantly improved after I58 pretreatment, and the distribution along the cell membrane is clearer, similar to the effect of using Lactobacillus plantarum (LP). IF method for F-actin det...

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Abstract

The invention discloses a protein sequence which has a sequence shown as a SEQIDNo.1 in an amino acid list. The protein sequence can be applied to medicines and medicaments for protecting enterocyte. A medicine containing the protein sequence is provided. The protein sequence in SLP originated from lactobacillus plantarum is applied to medicines and medicaments for protecting enterocyte. The medicine containing the sequence is further disclosed. The protein sequence has the positive effect of protecting enterocyte and has no side effect to human body.

Description

technical field [0001] The present invention relates to a protein sequence, especially a protein sequence of Lactobacillus plantarum and its application in medicine for protecting intestinal epithelial cells, and a medicine containing the sequence. Background technique [0002] The intestinal microbial ecosystem is huge in number and complex in composition, and the number of bacteria in it is about 10 times that of human somatic cells, which is closely related to the maintenance of human health and the occurrence of diseases. Among them, the adhesion and colonization of probiotics in the intestinal tract is of special significance to inhibit the colonization, translocation and infection of pathogenic bacteria, and maintain the integrity of the intestinal mucosa. In addition, with the extensive use of antibiotics, hormones, and chemical drugs, coupled with the influence of various toxic and harmful substances in food, the microbial flora in the human body has been severely da...

Claims

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Application Information

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IPC IPC(8): C07K14/335A61K38/16A61P1/00
Inventor 刘志华秦环龙汪建平
Owner 刘志华
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