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Breeding method for arginase transgenic corn

An arginase gene, corn breeding technology, applied in the directions of botanical equipment and methods, plant products, angiosperms/flowering plants, etc. The effect of increasing the level of aminidase

Inactive Publication Date: 2013-04-17
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0040] (2) Factors Affecting Agrobacterium Transformation
[0055] In addition to the above-mentioned main influencing factors, there are also some factors that also affect the transformation efficiency of transformed Agrobacterium

Method used

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  • Breeding method for arginase transgenic corn
  • Breeding method for arginase transgenic corn
  • Breeding method for arginase transgenic corn

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0075] 1 Vector plasmid and strain and recipient material:

[0076] The Agrobacterium strain used in the test is LBA4404, the basic expression vector pCAMBIA5300 (provided by the Chinese Academy of Agricultural Sciences, its full sequence is SEQ ID NO.2), and the promoter is Ubiquitin, which drives the expression of the ZmArg gene. The ubi promoter was used to drive the Bar gene as a selection marker, provided by the Chinese Academy of Agricultural Sciences.

[0077] 2 Experimental methods

[0078] 1. Construction of arginase gene ZmArg expression vector and Agrobacterium

[0079] (1) Amplify the ZmArg gene, the gene sequence is SEQ ID NO.1

[0080] (2) Using pCAMBIA5300-ubi-Tnos as the base vector, the HPT gene of the original vector was removed, and the 35S-driven CP4EPSPS gene from NK603 was replaced, and the arginase gene ZmArg, isopentenyl transferase gene ZmIPT2 and cell wall After the invertase gene ZmMn1 was inserted into the multiple cloning site SmaI, a monocot ov...

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Abstract

The invention discloses a breeding method for arginase transgenic corn, which comprises the steps of cloning ZmArg genes in the corn, converting an extremely early selfing line of the corn by an agrobacterium mediation method, and creating a high-yield new corn germplasm. With the method, the high-efficiency extraneous ZmArg genes are transplanted into the selfing line of the corn and expressed successfully; the arginase levels in various growth periods of the corn are raised to some extent; and yield traits such as thick corncobs, long niblets and wide niblets are correspondingly improved.

Description

technical field [0001] The invention belongs to the field of agricultural breeding, and in particular relates to a method for breeding corn with transarginase gene. Background technique [0002] Corn (Zea mays L.) is an important food and feed crop in the world, and it is also an important raw material for modern industry. With the development of industry and agriculture and the growth of world population, the demand for corn is increasing year by year. According to relevant studies, the world's corn demand will reach 753 million tons by 2020, and China's corn demand will be 214 million tons by then, which will make corn production face a huge challenge. However, the traditional breeding methods are faced with problems such as the promotion of single varieties, the narrowing of the genetic basis of breeding materials, and the increasingly poor gene pool. In addition, the conventional breeding cycle is long and predictable, and it is difficult to make crops more effective in...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00
Inventor 王振华邸宏曾兴张林刘显君翁建峰张佩
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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