Method for detecting lactic acid in fermented soybean meal
A technology for fermented soybean meal and determination method, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficulty in accurately measuring the lactic acid content in fermented soybean meal, no report on the lactic acid content in fermented soybean meal, and inaccuracy.
Inactive Publication Date: 2015-04-15
赵玉良
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Problems solved by technology
The study found that the neutralization titration method has a large error in the measurement results, and the titration result reflects the content of total acid, which is inaccurate; although the HPLC method can be qualitative and quantitative, the composition of fermented soybean meal is complex, containing protein, oligosaccharides, Small peptides, organic acids, isoflavones, etc. lead to large interference of miscellaneous peaks and unsatisfactory separation of main peaks when using HPLC to determine lactic acid. It is difficult to accurately determine the content of lactic acid in fermented soybean meal
There is no report on the determination method of lactic acid content in fermented soybean meal
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[0031] , reagents and materials
[0032] Unless otherwise specified, the reagents used in the present invention are analytically pure, and the water is ultrapure water.
[0033] 1.1 Methanol: chromatographically pure.
[0034] 1.2 Hydrochloric acid: chromatographically pure.
[0035] 1.3 0.01% hydrochloric acid solution: Accurately draw 0.1ml of hydrochloric acid (1.2), dissolve in water and dilute to 1000ml.
[0036] 1.4 Lactic acid standard: purity ≥ 99.99%.
[0037] 1.5 80% ethanol solution: Measure 800ml of absolute ethanol and add water to dilute to 1000ml.
[0038] 1.6 Lactic acid standard solution: Weigh 0.5000g of lactic acid standard product into a 100ml volumetric flask, dissolve it with 80% ethanol solution and dilute to the mark, and shake well. Each ml of solution contains 5 mg of lactic acid. After filtering through a 0.45um membrane filter, the filtrate was used for HPLC analysis.
[0039] 1.7 Fermented soybean meal: Connect soybean meal with lactic...
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The invention provides a method for detecting lactic acid in fermented soybean meal. The method for detecting lactic acid in fermented soybean meal, comprises the following steps of: (1) pre-treating a sample, (2) determining chromatogram conditions, (3) preparing a calibration curve, (4) determining the sample, and (5) calculating to obtain a result. The proteins in the fermented soybean meal are settled by 80% ethanol, macro-molecule substances in a supernatant is removed by filtrating with a 0.45mum filter membrane, the lactic acid in the fermented soybean meal is separated by a mixture mobile phase of methanol and hydrochloric acid, and the content of lactic acid is calculated by an external standard method. The sample is pretreated by a specific manner, the impurity interference can be avoided by selecting a proper mobile phase, and then the content of lactic acid in the fermented soybean meal can be accurately measured.
Description
technical field [0001] The invention relates to a method for measuring lactic acid content in fermented soybean meal. Background technique [0002] At present, soybean meal is mostly fermented with lactic acid bacteria, so fermented soybean meal contains a large amount of lactic acid and a small amount of propionic acid, butyric acid, etc. [0003] There are two commonly used methods for the determination of lactic acid: neutralization titration and high performance liquid chromatography. The study found that the neutralization titration method has a large error in the measurement results, and the titration result reflects the content of total acid, which is inaccurate; although the HPLC method can be qualitative and quantitative, the composition of fermented soybean meal is complex, containing protein, oligosaccharides, Small peptides, organic acids, isoflavones, etc. lead to large interference of miscellaneous peaks and unsatisfactory separation of main peaks when using H...
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IPC IPC(8): G01N30/02
Inventor 钟学敏罗富源张健龚音
Owner 赵玉良