Stem tip sterilizing primary culture method for germination accelerating for horseradish seeds
A technology of primary culture and horseradish, applied in the fields of seed and rhizome treatment, horticultural methods, botanical equipment and methods, etc., can solve problems such as untargeted removal of ink-borne diseases, and achieve suitable large-scale industrial production, germination and germination. High rate and the effect of overcoming technical bottlenecks
Active Publication Date: 2015-02-11
四川华阳蓝公府实业有限公司
View PDF2 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0005] There is currently no specific planting method for removing ink infestation
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreExamples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0026] (A) Germination: Select 500 seeds of Wasabi Tainong No. 2. The seeds are required to be plump and free from diseases and insect pests. First, use 0.2% mercury chloride for disinfection treatment. The treatment time is 8-10 minutes, and the seeds are kept at 18°C-22°C. The humidity is 90% to promote germination;
[0027] (B) When the germination length of wasabi seeds is 1-2cm, cut off the stem tip, the length is 0.3-0.4cm;
[0028] (C) Cleaning: After washing the sorted buds with washing powder, wrap them in gauze, rinse them with tap water for 2 hours, then soak them in 1% solution of Xiaoxiling for 30 minutes, and finally use sterile moisture-absorbing paper Basically absorb moisture;
[0029] (D) Sterilization: Soak the wasabi bud stem tips prepared in step (C) with 70% volume concentration of alcohol for 28-32 seconds, then disinfect with 0.2% mercury liter, and finally wash with sterile water for 5 times ;
[0030] (E) First-generation cultivation: The sterilize...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a germination accelerating and stem tip sterilizing primary culture method for horseradish seeds, and the culture method comprises the steps of (A) accelerating germination; (B) selecting materials; (C) cleaning: cleaning buds tidied by the step (B) with a washing powder solution, covering with gauze, continuously washing for 2 hours with running water, soaking for 30min with a 1% disinfectant solution, and basically absorbing water with sterile moisture absorption paper; (D) sterilizing: soaking horseradish bud stem tips prepared by the step (C) with alcohol which is 70% in volume concentration for 28-32s, disinfecting with 0.2% mercury bichloride, and cleaning for five times with sterile water; and (E) carrying out primary culture. According to the germination accelerating and stem tip sterilizing primary culture method for the horseradish seeds disclosed by the invention, through germination accelerating of the horseradish seeds and incising stem tips for culture, a 85% sterile seedling rate is achieved; and a following amplification test proves that the culture method is a significant breakthrough on the explant primary culture of the horseradish.
Description
technical field [0001] The invention relates to a method for cultivating seedlings in the field of agriculture, in particular to a method for cultivating wasabi seeds for germination and stem tip sterilization for primary generation. Background technique [0002] In the development of wasabi industry in our country, ink disease (also known as black heart disease) is the most important disease of wasabi planting, which can cause complete failure of wasabi planting in severe cases. Existing studies have found that squirrel disease is a specific disease of wasabi, which does not cause harm to other crops and does not cause adverse effects on human health. Ink disease is caused by the fungus Phoma wasabiae Yokogi invading wasabi plants and infecting wasabi plants mainly through vascular bundle conduction, which seriously affects the quality and yield of wasabi, and even leads to the failure of wasabi harvest. Ink disease can damage the rhizomes, fibrous roots, leaves, and petio...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01C1/00
Inventor 方季虎
Owner 四川华阳蓝公府实业有限公司