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Gulonic acid high-temperature fermentation method

A technology of high-temperature fermentation and gulong acid, which is applied in the direction of fermentation, can solve the problems of slowing down the fermentation speed due to the fermentation temperature, lengthening the fermentation cycle, and rising operating costs, so as to shorten the fermentation cycle, speed up the fermentation speed, and save fermentation refrigeration power fee effect

Active Publication Date: 2013-05-01
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lower fermentation temperature slows down the fermentation speed, prolongs the fermentation cycle, and reduces the fermentation yield; it also increases the burden on equipment cooling, especially in hot summer, in order to control the lower fermentation temperature, the refrigeration system needs to use expensive lithium bromide, etc. Refrigerant, so that the operating cost has risen sharply

Method used

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  • Gulonic acid high-temperature fermentation method

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Effect test

Embodiment 1

[0014] Gulonic acid high-temperature fermentation process in the present embodiment: the number of bacteria in 3ml is 6.4×10 8 Individual / ml, the seed solution that the ratio of small big bacterium count is 26: 1, inoculate into volume and be in the 250ml fermentation shaker flask, be filled with 20ml sorbose concentration and be 8%, the fermentation medium of pH 6.8 in the shaker flask, 29 ℃, shake (150 rev / min, dissolved oxygen percentage about 20%) and cultivate until the bacterial concentration reaches 7.2×10 8 cells / ml, increase the culture temperature to 42°C, and adjust the shaking frequency to 180 rpm (dissolved oxygen percentage is about 25%). After the 36th hour of fermentation, adjust the pH to 7.2-7.4 and ventilate at 150 rpm until the fermentation is complete to achieve high-temperature fermentation of gulong acid. The entire fermentation period is shortened by 17%, and the product yield is increased by 3.3%.

Embodiment 2-6

[0015] Embodiment 2-6 sees the following table

[0016]

[0017] *rpm: Shaker revolutions per minute.

[0018] The strains and culture medium described in the fermentation process of the above-mentioned vitamin C prerequisite for the production of gulong acid are all completed according to the records in the prior art.

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Abstract

The invention relates to the field of vitamin C two-step fermentation, and specifically relates to a gulonic acid high-temperature fermentation method. In a vitamin C production precondition which is gulonic acid fermentation process, a fermentation substrate is sorbose with a concentration of 8-9%, and an inoculum volume is 5-15%; when fermentation is started, a temperature is controlled at 29-31 DEG C, a pH value is 6.7-6.9, and a ventilation dissolved oxygen percentage is 20-22%; when fermentation bacteria concentration reaches 1-10*10<8> / ml, the temperature is gradually increased to 42-50 DEG C, and ventilation is increased, until mash dissolved oxygen percentage reached 25%; when fermentation is carried out for 40h, a pH value is regulated to 7.2-7.4, and dissolved oxygen percentage is reduced to 15%, until fermentation is finished, such that gulonic acid high-temperature fermentation is realized. According to the gulonic acid high-temperature fermentation method provided by the invention, fermentation speed is increased, fermentation period is shortened by 15-20%, and equipment utilization rate is improved by 10-15%.

Description

technical field [0001] The invention relates to the field of two-step fermentation of vitamin C, in particular to a high-temperature fermentation method of gulong acid. Background technique [0002] The two-step fermentation of Vc is a technology invented in the 1970s in my country, which is now adopted by domestic manufacturers. The main feature of this technology is that two kinds of bacteria are used for mixed fermentation, the substrate sorbose concentration in the fermentation is 8-9%, the fermentation temperature is 29-31°C, and the fermentation period is 55-60 hours. This technology has been used in production for more than 40 years and has met the general production requirements, but there are still many unsatisfactory places, one of which is that the fermentation temperature cannot be raised all the time. The lower fermentation temperature slows down the fermentation speed, prolongs the fermentation cycle, and reduces the fermentation yield; it also increases the b...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/60
Inventor 陈宏权徐慧吕淑霞安海英杨伟超邵立东姜铭妍宫英姿姚岚荆士华韩利涛朱葛张忠泽
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI