L-type PML/RAR alpha fusion gene detection kit and corresponding detection method

Abstract

The invention relates to the blood molecular biology field, and concretely relates to an L-type PML / RAR alpha fusion gene detection kit and a corresponding detection method. The L-type PML / RAR alpha fusion gene detection kit is composed of the following reagents of a functional oxidized grapheme, FITC-labeled single chain DNA with a sequence of 5'-FITC-TGACCTGCCATTGAG-3', a reagent I, formaldehyde, Escin and a PBS buffer, wherein the reagent I is composed of Tris alkali, NaCl, KCl, MgC12, HCl and H2O, an application method comprises the steps of cells culture, incubation liquid preparation by co-incubation with cells, and cells pretreatment and co-incubation of an incubation liquid and cells. The reagent provided by the kit can rapidly and accurately detect that whether certain cell exists the L-type PML / RAR alpha fusion gene or not, the kit has the advantages of sensibility, specificity, simple operation and rapidity, and the kit has important meaning for APL diagnosis and minimal residue monitoring, and the kit can realize recurrence prediction at early stage and clinical treatment guidance.

Description

Technical field [0001] The invention belongs to the field of blood molecular biology, and specifically relates to an L-type PML / RARα fusion gene detection kit and a corresponding detection method. Background technique [0002] Leukemia is a type of clonal malignant disease with abnormal hematopoietic stem cells. The leukemia cells in the clone lose the ability to further differentiate and mature and are stagnated at different stages of cell development. In the bone marrow and other hematopoietic tissues, leukemia cells proliferate and accumulate in large numbers and infiltrate other organs and tissues, while suppressing normal hematopoiesis. The clinical manifestations are symptoms of anemia, bleeding, infection and infiltration of various organs. Chromosomal abnormalities are one of the common causes of leukemia. According to the maturity of leukemia cells and the natural course of the disease, leukemia can be divided into acute and chronic categories. The differentiation of ...

AI Technical Summary

Problems solved by technology

[0003] At present, the detection methods of PML / RARα fusion gene mainly include chromosome analysis, real-time quantitative RT-PCR, fluorescence in situ hybridization, etc., but these methods have problems such as low specificity, cumbersome operation, and high detection cost, which limit their wide application. [Wei Na, Chen Jinghua, Wang Kun, et al. Research on the Electrochemical Sensor of Hairpin Structure DNA Probes for Detection of Leukemia PML / RARα Fusion Gene[J]. Journal of Analysis and Testing, 2008, 27(9): 907-910]

Images