Production method and application of patterned biochemical analysis test paper

Abstract

The invention discloses a production method and an application of patterned biochemical analysis test paper. The test paper is of a compound structure comprising wax and absorbent paper. The production method comprises the steps of: melting the solid wax into liquid on heating equipment; immersing a metal template with a required pattern into the liquid wax to further heat for 5-10 minutes; printing the metal template with the liquid wax as a stamp on the surface of the absorbent paper; absorbing the liquid wax on the metal template by the absorbent paper; cooling to form a plurality of millimeter-sized hydrophilic channels taking the wax as a hydrophobic barrier; and adding a biochemical analysis detection reagent in a set reaction area to obtain the patterned biochemical analysis test paper. The patterned biochemical analysis test paper is applied to multi-index biochemical analysis of various liquid samples such as urine, blood, serum, saliva and water; and the production method has the advantages of low cost and the like, and is simple and quick to operate.

Description

technical field [0001] The invention relates to the development and application of biochemical analysis products, and in particular provides a patterned biochemical analysis test paper, its manufacturing method and application. Background technique [0002] As a low-cost and easy-to-operate analysis method, test strips have been widely used in real life, for example, pregnancy test strips, pH test strips, urine test strips, etc. Compared with the traditional production method of biochemical analysis test paper used for detection of multiple indicators, the present invention is one-time forming, simple in operation, less time-consuming and low in cost, and is especially suitable for various small-scale laboratories and on-site production. The patterned biochemical analysis test paper is also called Paper-based microfluidics or Lab-on-paper. [0003] At present, the production methods of patterned biochemical analysis test paper reported in the literature mainly include photo...

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Problems solved by technology

[0003] At present, the production methods of patterned biochemical analysis test paper reported in the literature mainly include photolithography, paraffin printing, drawing, etc., and their main limitation is the instruments involved (for example, photolithography, wax printer, mechanical drawing instrument, etc.) ) and materials (for example, photoresist SU8, printer-specific paraffin, polydimethylsiloxane, etc.) are extremely expensive and uncommon, and the operation steps are relatively cumbersome and time-consuming, and require high professional skills for operators, so It is not suitable for making patterned biochemical analysis test paper in various small laboratories or in the field, which limits the wide application of this new multi-index biochemical analysis test paper in many fields (Patterned Paper as a Platform for Inexpensive, Low-Volume, Portable Bioassays. Andres W. Martinez, Scott T. Phillips, Manish J. Butte and George M. Whitesides. Angew. Chem. Int. Ed. 2007, 46, 1318–1320; Fabrication and Characterization of Paper-Based Microfluidics Prepared in Nitrocellulose Membrane By Wax Printing . Yao Lu, Weiwei Shi, Jianhua Qin and Bingcheng Lin. Anal. Chem. 2010, 82, 329–335; Low-Cost Printing of Poly(dimethylsiloxane) Barriers To Define Microchannels in Paper Derek A. Bruzewicz, Meital Reches, and George M. Whitesides. Anal. Chem. 2008, 80, 3387-3392)

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