Method for distinguishing degree of production traits of spirulina strain

A technology for producing traits and spirulina, which is applied in the direction of analyzing materials, analyzing materials through optical means, measuring devices, etc., can solve the problems of high cost, cumbersome procedures, and long cycle, and achieve the effect of low cost

Inactive Publication Date: 2013-06-12
ZHEJIANG UNIV
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Although this method is practical and effective, the procedure is cumb

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  • Method for distinguishing degree of production traits of spirulina strain
  • Method for distinguishing degree of production traits of spirulina strain
  • Method for distinguishing degree of production traits of spirulina strain

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[0022] Sodium dodecylsulfonate-polyacrylamide gel electrophoresis (SDS-PAGE) technology of protein has been widely used in the classification and identification of tobacco, rice, woody plants and seaweed, etc. However, there are few reports on the application of SDS-PAGE fingerprints and related analysis methods in classification and identification. The invention establishes a new method for detecting the optical density value of the band at 102kD in the protein fingerprint, and identifying the excellent production properties of spirulina and whether it can be applied to large-scale production and breeding through clustering.

[0023] We used 9 well-known strains of Spirulina Sp-1, Sp-2, Sp-4, Sp-5, Sp-6, Sp-9, Sp-10, Sp-15 and Sp-17 as control materials. SDS-PAGE and Quantity One software were used to detect the size of the optical density value of the protein band at 102kD in the protein electrophoresis spectrum, and cluster analysis was performed according to its size. It w...

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Abstract

The invention relates to a spirulina development and application technology and aims to provide a method for distinguishing the degrees of production traits of a spirulina strain. The method provided by the invention comprises the following steps of: extracting water-soluble protein of spirulina cells with an tris-HCl extracting solution, separating a protein sample by carrying out sodium dodecyl sulphate-polyacrylamide gel electrophoresis, then detecting the light intensity value of a protein band at a 102kD site of a protein electrophoretogram, and constructing a hierarchical diagram among algae plants according to the light intensity value; if the light intensity value of the protein band of a candidate strain at the 102kD site is more than 140 and the candidate strain and a known strain with good production traits get together, indicating that the candidate strain has good production traits and applicable to large-scale cultivation production; and if the light intensity value of the protein band of the candidate strain at the 102kD site is less than 40 and the candidate strain and a known strain with poor production traits get together, indicating that the candidate strain has bad production traits and is not applicable to large-scale cultivation production. The method provided by the invention is simple, efficient, reliable and low in cost, and no complex test and analysis such as nucleic acid sequencing and bioinformatics comparison is carried out, so that the method provided by the invention is applicable to large-scale high throughput screening.

Description

technical field [0001] The invention belongs to the technology for the development and application of spirulina, in particular to a method for distinguishing the pros and cons of production traits of spirulina strains. Background technique [0002] Spirulina is a photosynthetic, oxygen-releasing, regular spiral prokaryotic filamentous microalgae, a genus of Cyanophyta, Oscillatoriales, and Oscillatoriaceae [Wuhan Botany Research, 1997, 15(4): 369-374]. Because it is rich in high-quality protein and a variety of biologically active substances, it has attracted great attention at home and abroad, and has formed a huge spirulina industry on the basis of a large number of researches, becoming the world's largest research and development scale and the most extensive application prospects. microalgae. It is worth pointing out that numerous experimental studies and long-term production practices have shown that different varieties (strains) of Spirulina have different requirement...

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Application Information

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IPC IPC(8): G01N21/84
Inventor 汪志平于金鑫刘新颖吕蓓芬马丽芳陈子元
Owner ZHEJIANG UNIV
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