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An immunomodulator composition, and a pharmaceutical composition and applications thereof

A technology of immunomodulator and composition, applied in the field of immunomodulator composition and its pharmaceutical composition, can solve the problems of insignificant therapeutic effect and long-lasting immunomodulatory effect, and reduce the outbreak and pandemic of infectious diseases Risk, the effect of enhancing the ability of non-specific immune response

Active Publication Date: 2013-06-19
云帆医学科技(广州)有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is also found that its immunomodulatory effect is not durable, and its therapeutic effect is not significant

Method used

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  • An immunomodulator composition, and a pharmaceutical composition and applications thereof
  • An immunomodulator composition, and a pharmaceutical composition and applications thereof
  • An immunomodulator composition, and a pharmaceutical composition and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the cultivation of BCG and the preparation of polysaccharide extract

[0039] 1. Bacterial culture: Dissolve the strains preserved in liquid cryogenic temperature (BCG strain D2PB302 for the preparation of BCG in China, provided by the Bacteria Room of China Institute for the Control of Pharmaceutical and Biological Products) at room temperature, inoculate them in Potato Sutong medium, and culture continuously at 37°C 14-20 days; or after continuous cultivation at 37°C for 15 days, transfer to improved liquid Sutong medium, and continuous cultivation at 37°C for 14-20 days.

[0040] Wherein, the preparation method of potato Sutong culture medium can be:

[0041] Take washed fresh potatoes (1) and puncture them into cylinders with a piercer, then cut them into 4 cm long slopes with a knife, rinse the potato slopes with running drinking water for 1 hour, and then rinse the potato slopes with purified water; then use Sutong culture medium Rinse the ramp bloc...

Embodiment 2

[0064] Embodiment 2: the cultivation of pneumococcus and the extraction of polysaccharide

[0065] 1. Cultivation of pneumococci: Culture bottles and fermenters can be used for cultivation, and the cultivation conditions are the pneumococcus cultivation conditions known in the art.

[0066] Inoculate 1, 5, 6B, 14, 18C, 19F and 23F serotype freeze-dried strains of pneumococcus on 10% defibrated sheep blood common agar medium, at 36°C, 5% CO 2 In the environment, the 2nd generation liquid medium is propagated after static culture, and the inoculum size is about 10 8 CFU / mL was inoculated in a fermenter with a certain volume, and cultured with stirring at 36°C. After 2 hours of culture, samples were taken every 1 hour, and the OD value was measured at a wavelength of 600nm. mol / L NaOH was used to adjust the pH to 7.5, and compressed air was fed intermittently, and the culture was terminated when the OD value reached 1.5-1.7 (about 7-9 hours). Bacteria were inactivated with (V / V...

Embodiment 3

[0071] Embodiment 3: the cultivation of staphylococcus aureus and the extraction of polysaccharide

[0072] 1. Cultivation of Staphylococcus aureus: Culture bottles and fermenters can be used for cultivation, and the cultivation conditions are the pneumococcus cultivation conditions known in the art.

[0073] Inoculate the freeze-dried strains of Staphylococcus aureus of C5 and C8 serotypes on solid medium for activation, culture at 37°C for 18 hours, and culture the culture on new agar culture at 37°C for 4 hours in order to obtain the exponential growth phase of microorganisms. Then in basic liquid medium (1g / L glucose, 120mg / L Fecl 3 ·6H 2 O, 400mg / LMgcl 2 ·6H 2 O, 10mg / L CaCl 2 2H 2 O, 5mg / LZnCl 2 , 58.5g / L NaCl, 6g NH 4 Cl, 6.8g NH 4 Cl, pH6.2~7.5, optimally 6.5) for cultivation. After culturing for 2 hours, samples were taken every 1 hour, and the OD value was measured at a wavelength of 600nm. 600 When ≥1.5, terminate the culture.

[0074] The culture was in...

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Abstract

The present invention relates to an immunomodulator composition, and a pharmaceutical composition and applications thereof. The immunomodulator composition consists of a variety of bacterial polysaccharide extracts, wherein at least a bacterial polysaccharide extract derived from mycobacterium and a polysaccharide extract derived from pneumococcus are comprised. The immunomodulator composition can stimulate a body to produce a sustained immune function of a significant effect, particularly non-specific immune response ability as well as secondary generated specific immune response on the basis thereof. Compared with immunomodulators already available in the market, the immunomodulator composition of the invention can improve the overall anti-infection, anti-tumor and anti-allergic abilities for human or animals.

Description

technical field [0001] The present invention relates to a composition, in particular to an immunomodulator composition and a pharmaceutical composition thereof. The immunomodulator composition is composed of various bacterial polysaccharide extracts, at least including the polysaccharide extracts derived from mycobacterium bacteria and pneumococcus polysaccharide extracts. Background technique [0002] The decline of human immune function is one of the important reasons leading to the infection of pathogenic organisms such as bacteria and viruses. Nonspecific immune response (also called innate immune response) is the first line of defense against pathogenic organisms in humans and vertebrates. Recognition molecules (pathogen associated molecule patterns, PAMPs) recognize and start a non-specific immune response, and then trigger a specific immune response (also known as an acquired immune response) to eliminate pathogenic organisms and protect the body. [0003] Nonspecif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K31/715A61P37/02A61P37/04A61P37/08A61P31/00A61P35/00A61P11/06A61P11/00
CPCY02A50/30
Inventor 宁云山
Owner 云帆医学科技(广州)有限责任公司
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