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Method for measuring content of pyrroloquinoline quinone dimethyl ester by micellar electrokinetic chromatography

A technology of quinoline quinone dimethyl ester and capillary electrophoresis, which is applied in the field of chromatographic analysis, can solve problems such as difficult separation, and achieve the effects of low mass detection limit, reduced velocity gradient, and low reagent consumption

Inactive Publication Date: 2014-08-13
JIANGSU INST OF NUCLEAR MEDICINE
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Problems solved by technology

PQQZ has electrical neutrality, and it is difficult to achieve ideal separation by ordinary high-performance liquid chromatography and other analytical methods.

Method used

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  • Method for measuring content of pyrroloquinoline quinone dimethyl ester by micellar electrokinetic chromatography
  • Method for measuring content of pyrroloquinoline quinone dimethyl ester by micellar electrokinetic chromatography
  • Method for measuring content of pyrroloquinoline quinone dimethyl ester by micellar electrokinetic chromatography

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Embodiment 1

[0026] (1) Capillary electrophoresis analysis conditions:

[0027] Uncoated quartz capillary column (42cm×50μm, Hebei Yongnian Ruifeng Chromatography Device Co., Ltd.), the detection wavelength is 250nm, the injection method is 0.5psi pressure injection, the injection time is 10.0 seconds, the separation voltage is 22kv, the capillary column Temperature 25°C, running buffer solution: 50mmol / L disodium hydrogen phosphate-50mmol / L borax-10mmol / L sodium dodecyl sulfate (SDS) mixture, pH=7.0, containing 10% acetonitrile. Both the buffer and the sample solution were filtered with a 0.22 μm microporous syringe filter. Rinse the new capillary with methanol for 10 minutes, distilled water for 5 minutes, 1mol / L NaOH solution for 30 minutes, distilled water for 5 minutes, and finally run buffer for 10-15 minutes. Before each injection, rinse with distilled water and running buffer for 2 minutes.

[0028] (2) Selection of buffer system

[0029] The buffer system and concentration dire...

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Abstract

The invention provides a method for measuring the content of pyrroloquinoline quinone dimethyl ester by micellar electrokinetic chromatography and belongs to the technical field of chromatographic analysis. Many nervous system degenerative diseases are related to D-serine metabolism. The chemical reaction of the pyrroloquinoline quinone dimethyl ester (PQQZ) and D-serine is analyzed so as to analyze whether PQQZ has the same regulation effect on amino acids and know whether PQQZ has protection effect on nerve cells; therefore, experiment basis is provided for drug design. The method provided by the invention employs the micellar electrokinetic chromatography (MECC) for the first time; the surfactant lauryl sodium sulfate (SDS) is added to a buffer liquid system to form a micelle, and an organic modified reagent is added so that PQQZ is effectively and quickly separated from other electrically neutral impurities different to separate; and the purity of the pyrroloquinoline quinone dimethyl ester is analyzed. The micellar electrokinetic chromatography (MECC) is extremely low in quality detection limit in contrast with other methods, and the dosage of the sample is only nL-level and the consumption of the reagent is also low.

Description

technical field [0001] The invention discloses an analysis method for determining the content of pyrroloquinoline quinone dimethyl ester by micellar electrokinetic capillary electrophoresis, which belongs to the technical field of chromatographic analysis. Background technique [0002] A large number of studies have shown that in animals, prion disease is mainly concentrated in the transformation process of benign cellular protein PrP-sen to viral protein PrP-res, and the process of protein structure transformation is accompanied by the death of neurons, causing irreversible and fatal damage to the central nervous system. neurodegenerative disease. [0003] It has been reported in the literature that electron-rich aromatic and polyanionic compounds can inhibit the replication of the pathogenic protein PrP-res by capturing prion free radicals or forming ionic bonds with oxygen-containing reaction intermediates, and thus become potential drugs for brain and mental diseases. A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/416
Inventor 周杏琴毛师师张建康
Owner JIANGSU INST OF NUCLEAR MEDICINE
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