Method for measuring content of pyrroloquinoline quinone dimethyl ester by micellar electrokinetic chromatography
A technology of quinoline quinone dimethyl ester and capillary electrophoresis, which is applied in the field of chromatographic analysis, can solve problems such as difficult separation, and achieve the effects of low mass detection limit, reduced velocity gradient, and low reagent consumption
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[0026] (1) Capillary electrophoresis analysis conditions:
[0027] Uncoated quartz capillary column (42cm×50μm, Hebei Yongnian Ruifeng Chromatography Device Co., Ltd.), the detection wavelength is 250nm, the injection method is 0.5psi pressure injection, the injection time is 10.0 seconds, the separation voltage is 22kv, the capillary column Temperature 25°C, running buffer solution: 50mmol / L disodium hydrogen phosphate-50mmol / L borax-10mmol / L sodium dodecyl sulfate (SDS) mixture, pH=7.0, containing 10% acetonitrile. Both the buffer and the sample solution were filtered with a 0.22 μm microporous syringe filter. Rinse the new capillary with methanol for 10 minutes, distilled water for 5 minutes, 1mol / L NaOH solution for 30 minutes, distilled water for 5 minutes, and finally run buffer for 10-15 minutes. Before each injection, rinse with distilled water and running buffer for 2 minutes.
[0028] (2) Selection of buffer system
[0029] The buffer system and concentration dire...
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