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Coated beads

A coating and molecular technology, applied in instruments, microcapsules, capsule delivery, etc., can solve the problems of reduced and completely denatured proteins lacking tertiary and secondary structures

Inactive Publication Date: 2013-06-19
THE BINDING SITE GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A fully denatured protein that lacks tertiary and secondary structure and thus loses or reduces its ability to bind or be bound by the corresponding reactant in an immunoassay

Method used

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  • Coated beads
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Examples

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Embodiment Construction

[0050] Coating of beads is conventional and well known in the art. That is, beads such as polystyrene beads are coated with an appropriate buffer, such as phosphate-buffered saline pH 7.2 or carbonate pH 9.6, which contains a typical concentration of 0.5-20ug / mL (depending on the protein) desired antigen or antibody and mixed gently at room temperature for 8-12 hours. The volume of buffer equals 50 μL / bead, so for 100,000 beads this equals 5000 mL. Excess coating solution is then usually aspirated off. Subsequent additions usually contain 50% Stabilcoat TM or Stabilguard TM of blocking / stabilizing buffer and mix for 30 min at room temperature, again using 50 μL / bead. Then discard most of the remaining blocking / stabilizing buffer. Typically, however, a small percentage of liquid remains, such as 2-5%. This was found to be necessary to allow the physical layer of the stabilizer to form a protective shell over the bound antigen / antibody. The beads and remaining liquid are ...

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Abstract

The invention relates to a method of coating beads with a biological molecule comprising: (i) coating a plurality of beads with the biological molecule; (ii) mixing the coated beads with a liquid stabilising and / or blocking agent (iii) dispersing the coated beads still substantially surrounded by a liquid phase comprising the liquid stabilising and / or blocking agent across a surface that is at least partially liquid permeable; (iv) drying the beads on the surface to substantially remove the liquid phase; and (v) removing the dried beads from the surface.

Description

technical field [0001] The present invention relates to methods of coating beads with biological molecules and beads coated with biological molecules. Background technique [0002] Diagnostic immunoassays such as enzyme-linked immunosorbent assay (ELISA) 1,2 Widely used in research and industry and is an important and sensitive analytical technique for many diagnostic, clinical, biochemical, environmental and genetic applications 3,4 . These assays use proteins (e.g., antigens and antibodies) immobilized on solid supports and labeled with chromogenic, fluorescent, radioactive, and chemiluminescent molecules and / or with enhancers or substrates, including enzymes, that serve as reporters. Detection proteins conjugated to other biomolecules. [0003] In order to successfully manufacture immunoassay solid supports with extended shelf life greater than days / weeks, it is necessary to maintain the physical integrity of the immobilized target antigen or antibody. This is affecte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/543A61K9/20
CPCA61K9/5078G01N33/5306G01N33/54393G01N33/54313
Inventor R·布德D·泰勒
Owner THE BINDING SITE GROUP
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