Process for extracting Penis et Testis Canis polypeptide through enzymatic method, and obtained Penis et Testis Canis polypeptide extract
A technology of enzymatic extraction and extraction, which is applied in the field of dog kidney deep processing, can solve the problems of cumbersome process, high extraction cost, unfavorable industrial production and preparation, etc., and achieve the effect of high extraction rate, reduced solvent consumption, and full and comprehensive active ingredients
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Embodiment 1
[0033] See figure 1 As shown in the extraction process, the enzymatic extraction of dog kidney polypeptide in this embodiment is mainly carried out as follows:
[0034] Weigh 100g of animal medicinal dog kidney, wash, soak until softened, minced with a meat grinder, pass through an 80-mesh stainless steel screen, add 400mL of water, add papain (specification 200,000 U / g, Guangbo Nanning Pangbo Bioengineering Co., Ltd. Produced by the company) 2g, trypsin (specification 250,000 U / g, produced by Shanghai Sangon Bioengineering Co., Ltd.) 1g, after fully stirring evenly, enzymolyze at 55°C for 2 hours, add 200mL of ethanol with a volume concentration of 95%, and prepare into ethanol extract, heat at 90°C under normal pressure and reflux for 3 hours, filter the extract with a 100-mesh filter cloth, and remove the liquid surface grease; add 800mL of ethanol with a volume concentration of 60% to the residue, heat and boil under normal pressure for 2 hours, and filter through a 100-me...
Embodiment 2
[0037] Weigh 100g of dog kidney, an animal medicinal material, wash, soak until soft, mince with a meat grinder, pass through an 80-mesh stainless steel sieve, add 400mL of water, add trypsin (specification 250,000 U / g, Shanghai Sangon Bioengineering Co., Ltd. production) 3g, after fully stirring evenly, enzymatically hydrolyze at 55°C for 2 hours, add 200mL of 95% volume concentration of ethanol to make ethanol extract, heat at 90°C under normal pressure for 3 hours, filter with 100-mesh filter cloth to obtain the extract , to remove the liquid surface grease; add 800mL of ethanol with a volume concentration of 60% to the residue, heat and boil under normal pressure for 2 hours, filter with a 100-mesh filter cloth to obtain the extract, combine the two ethanol extracts with a rotary evaporator (at a temperature of 70°C) ) is concentrated into an extract, ethanol is recovered during the concentration process, and finally the extract is vacuum-dried below 60° C., and the obtaine...
Embodiment 3
[0040] Weigh 100g of animal medicinal dog kidney, wash, soak until softened, minced with a meat grinder, pass through an 80-mesh stainless steel screen, add 400mL of water, add papain (specification 200,000 U / g, Guangbo Nanning Pangbo Bioengineering Co., Ltd. Produced by the company) 3g, after fully stirring evenly, enzymatically hydrolyze at 55°C for 2 hours, add 200mL of ethanol with a volume concentration of 95% to make ethanol extract, heat at 90°C under normal pressure for 3 hours, filter with 100 mesh filter cloth to obtain the extract Add 800mL of ethanol with a volume concentration of 60% to the residue, boil and reflux for 2 hours under normal pressure, filter with a 100-mesh filter cloth to obtain the extract, combine the two alcohol extracts and use a rotary evaporator (70°C) temperature) concentrated into an extract, ethanol is recovered during the concentration process, and finally the extract is vacuum-dried below 60° C., and the obtained black-brown dry extract i...
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