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A kind of microbial strain and application for natural gas purification

A technology of microbial strains and natural gas, applied in the directions of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve problems such as difficult to achieve purification rate

Active Publication Date: 2015-08-19
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously for the acidic H 2 As far as S gas is concerned, it is difficult to achieve a satisfactory purification rate in natural gas desulfurization
Therefore, there is no report of this kind of strain technology in natural gas biological desulfurization

Method used

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  • A kind of microbial strain and application for natural gas purification
  • A kind of microbial strain and application for natural gas purification
  • A kind of microbial strain and application for natural gas purification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Isolation and identification of bacterial classification

[0018] Collect activated sludge from the sewage treatment section of a production operation area in Southwest Oil and Gas Field. Through acclimatization and enrichment, take 1 mL of the acclimatized bacterial solution, and use sterile water to make dilutions with concentrations of 10-1, 10-2, 10-3, 10-4, 10-5, and 10-6 in sequence. Then inoculate 0.2mL of 10-4, 10-5, and 10-6 dilutions on the plate medium respectively, spread evenly, and place them upside down in a biochemical incubator for cultivation until white circles appear on the surface of the solid medium. Point-like small colonies, this process takes 10-14d.

[0019] Stir up the white single colony and burn it on the glass slide, and there will be obvious sulfur smell. Observe the bacteria under a microscope, the size of the bacteria is 0.5-2.5 μm, single, in pairs, or arranged in short chains, crystals can be seen in the cells under the...

Embodiment 2

[0020] Embodiment 2: The influence of initial pH value on the growth of strain TYY-1

[0021] The growth of TYY-1 was investigated under the conditions of initial pH values ​​of 5.0, 6.0, 7.0, 8.0, 9.0 and 10.0. The results showed that the OD value of Thiobacillus TYY-1 was the highest at 8.0, indicating the best microbial growth.

[0022] attached image 3 .

Embodiment 3

[0023] Embodiment 3: the influence of temperature on the growth of bacterial classification TYY-1

[0024] The growth of TYY-1 was investigated at 20°C, 25°C, 30°C, 35°C and 40°C. The results showed that the OD value of Thiobacillus TYY-1 was the highest at 30°C, indicating that the microorganisms grew best. attached Figure 4 .

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Abstract

The invention relates to a microbial strain used for purifying natural gas and an application. The strain belongs to Thiobacillus, and is named as Thiobacillus sp. TYY-1. and is preserved in a preservation center of type culture objects in China (CCTCC), and the strain number is CCTCC NO: M2010282; the temperature scope for growing the microbial strain is 15-45 DEG C, the pH scope for growth is 6.0-9.0, the inoculation amount during an enlargement culture process is 10%; the strain, successive strains, variant strains and derivative strains which keep the active strain can be used in natural gas purifying treatment; hydrogen sulfide concentration in raw material gas is lower than 40g / m<3>, the hydrogen sulfide removal rate by the strain can reach 100%; hydrogen sulfide concentration in raw material gas is greater than 80g / m<3>, the hydrogen sulfide removal rate by the strain can reach 95%; the microbial strain has high purifying capability on hydrogen sulfide in natural gas.

Description

technical field [0001] The invention relates to a microbial strain for natural gas purification and its application, as well as the application of the subculture strain, variant strain and derivative strain retaining the active strain in natural gas desulfurization. Background technique [0002] Judging from the current domestic natural gas exploration and development status, more and more scattered sulfur-containing natural gas needs to be processed. From the perspective of comprehensive evaluation of economic indicators and treatment effects, when the latent sulfur content is less than 0.1t / d, dry desulfurization is more suitable; when the latent sulfur content is greater than 50t / d, the amine method + Claus process can fully reflect the economic benefits; When the latent sulfur content is between the two, the current ideal way at home and abroad often adopts liquid phase redox desulfurization technology for treatment. However, the main disadvantages of liquid-phase redox...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C10L3/10C12R1/01
Inventor 徐波常宏岗何金龙温崇荣杨芳刘其松孙茹
Owner PETROCHINA CO LTD