Method for preparing sugar grass seed protein ACE (Angiotensin Converting Enzyme) inhibitory peptide through binary-enzyme step hydrolysis, and product and application of sugar grass seed protein ACE inhibitory peptide
A step-by-step hydrolysis and sweet sorghum technology, applied in the field of bioengineering, can solve the problems of mixed enzyme hydrolysis interference, insufficient single-enzyme hydrolysis sites, ineffective release of ACE inhibitory peptides, etc., and achieves improved inhibitory activity, stable product properties, and improved operation. simple effect
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Embodiment 1
[0026] Make the crushed sweet sorghum seed powder into 5% (w / v) suspension, use amylase to enzymolyze at 50°C for 3 hours, and add enzyme at 5000U / g, after the enzymolysis, adjust the pH of the suspension to 8.0, stir for 30 minutes and then centrifuge, take the supernatant, adjust its pH to 4.0, let it stand for 30 minutes, then centrifuge, collect the precipitate, dissolve the precipitate in distilled water, adjust the pH to neutral and then spray dry to obtain sweet sorghum seed protein powder .
[0027] Take 20g of sweet sorghum seed protein powder and prepare a solution with a mass percentage of 1% (w / v), adjust the pH to 7.0, use α-chymotrypsin to enzymolyze at 40°C for 20min, and add enzyme at 2000U / g. After the hydrolysis, the enzyme was deactivated in a boiling water bath, the pH of the reaction mixture was adjusted to 6.0, and the enzyme was hydrolyzed at 50°C for 40 minutes with neutral protease, and the amount of enzyme added was 2000 U / g. The supernatant and supe...
Embodiment 2
[0029] Make 8% (w / v) suspension of crushed sweet sorghum seed powder, use amylase to hydrolyze at 60°C for 3.5 hours, and add 7500 U / g of enzyme, after the hydrolysis, adjust the pH of the suspension to to 9.0, stirred for 30 minutes, centrifuged, took the supernatant, adjusted its pH to 4.2, let it stand for 30 minutes, centrifuged, collected the precipitate, dissolved the precipitate in distilled water, adjusted the pH to neutral, and then spray-dried.
[0030] Take 20g of sweet sorghum seed protein powder and prepare a solution with a mass percentage of 2% (w / v), adjust the pH to 7.5, use α-chymotrypsin to enzymolyze at 45°C for 25min, and add enzyme at 3000U / g. After the hydrolysis, the enzyme was deactivated in a boiling water bath, the pH of the reaction mixture was adjusted to 6.5, and the enzyme was hydrolyzed at 55°C for 50 min with neutral protease, and the amount of enzyme added was 3000 U / g. The supernatant and supernatant were concentrated to a solid content of ab...
Embodiment 3
[0032] Make 10% (w / v) suspension of crushed sweet sorghum seed powder, use amylase to enzymatically hydrolyze at 70°C for 4 hours, the amount of enzyme added is 10000U / g, after the enzymatic hydrolysis, adjust the pH of the suspension to 9.5, centrifuge after stirring for 30 minutes, take the supernatant, adjust its pH to 4.5, let it stand for 30 minutes, then centrifuge, collect the precipitate, dissolve the precipitate in distilled water, adjust the pH to neutral, and then spray dry.
[0033] Take 20g of sweet sorghum seed protein powder to prepare a solution with a mass percentage of sorghum seed protein powder of 4% (w / v), adjust the pH to 8.0, and use α-chymotrypsin to enzymolyze at 50°C for 30 minutes, and the amount of enzyme added is 4000U / g, after the enzymolysis is completed, the enzyme is killed in a boiling water bath, the pH of the reaction mixture is adjusted to 7.0, and the enzyme is hydrolyzed at 60°C for 60 minutes with neutral protease, the amount of enzyme a...
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