A kind of identification method of Radix Bupleurum medicinal material
An identification method and technology of medicinal materials, applied in the direction of material separation, analysis of materials, measuring devices, etc., can solve problems such as adverse consequences
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Embodiment 1
[0049] A method for identification of Bupleurum medicinal material, characterized in that the method comprises the following steps:
[0050] Step 1, preparation of reference substance solution;
[0051] Step 2, the preparation of need testing solution;
[0052] Step 3, injecting the solutions described in step 1 and step 2 into the HPLC chromatograph respectively to obtain a chromatogram;
[0053] Step 4, comparing the similarity of the chromatograms, the similarity shows that the test product is qualified.
[0054] Wherein, the preparation of step 1 reference substance solution is as follows: take a certain amount of saikosaponin a reference substance, accurately weigh it, put it in a 10ml measuring bottle, add methanol to dilute to the mark, shake well, and get 0.1mg / ml saikosaponin a solution. Among them, the preparation of the test solution in step 2, the method is as follows: take 4 g of Bupleuri herb, add 50 ml of water, reflux extraction, the first time for 1 hour, t...
Embodiment 2
[0064]A standard fingerprint of Bupleurum medicinal material is determined according to the following method: take a certain amount of saikosaponin a reference substance, weigh it accurately, put it in a 10ml measuring bottle, add methanol to dilute to the mark, and shake well to get 0.1mg / ml bupleurum. Saponin a solution, the solution is injected in the HPLC chromatograph, obtains chromatogram; Chromatographic condition is as follows:
[0065] Chromatographic column: ZorbaxSB-C18 column (250mm×4.6mmID, 5μm, Agilent)
[0066] Mobile phase: acetonitrile: water (containing 0.05% phosphoric acid) gradient elution as follows:
[0067]
[0068] Detection wavelength: 220nm
[0069] Flow rate: 1.0mL·min-1
[0070] Column temperature: 25°C
[0071] Injection volume: 10 μL.
Embodiment 3
[0073] The fingerprint of a kind of Bupleurum medicinal material is determined according to the following method: take 4 g of Bupleurum medicinal material, add 50 ml of water, reflux extraction, the first time for 1 hour, the second time for 1 hour, filter, combine the filtrates, concentrate to an appropriate amount, add 95% Alcohol precipitation, overnight, suction filtration, after the filtrate was concentrated under reduced pressure, it was fixed to a 25ml measuring bottle, and the solution was injected into the HPLC chromatograph to obtain a chromatogram; the chromatographic conditions are as follows:
[0074] Chromatographic column: ZorbaxSB-C18 column (250mm×4.6mmID, 5μm, Agilent)
[0075] Mobile phase: acetonitrile: water (containing 0.05% phosphoric acid) gradient elution as follows:
[0076]
[0077] Detection wavelength: 220nm
[0078] Flow rate: 1.0mL·min-1
[0079] Column temperature: 25°C
[0080] Injection volume: 10 μL.
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