Preparation method of acid soluble soy protein

A soy protein and acid technology, which is applied in the directions of protein food ingredients, protein food processing and application, to achieve the effects of improved taste, high yield and simple steps

Inactive Publication Date: 2013-07-17
EAST CHINA NORMAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this acidic protein drink made of only phytase-treated soy protein will form a large amount of sediment after standing for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0012] The preparation method of acidic soluble soybean protein of the present invention specifically comprises the following steps:

[0013] (1) Alkali dissolution: Add 10 times the volume of water to the low-temperature defatted soybean meal at a temperature of 40-50°C, adjust the pH to 7-9 with sodium hydroxide solution, stir for 30-60 minutes, and centrifuge at 4000g to remove the soybean dregs to obtain soybean protein Liquid.

[0014] (2) Add phytase to the soybean protein feed liquid, the amount added is 10-100u / g calculated according to the protein content of the feed liquid, preferably 50-100u / g; stir at 35-45°C for 30-60min , preferably stirring at 40-45°C for 45min.

[0015] (3) Make the feed liquid pass through a filter screen with a pore size of 200 to avoid clogging of the ultrafiltration membrane.

[0016] (4) Pass the feed liquid through an ultrafiltration membrane with a molecular cut-off of 5,000-30,000 Daltons for ultrafiltration. The soybean protein feed...

Embodiment 1

[0020] Add 3L of water to 300g of low-temperature defatted soybean meal (purchased from Xuchang Bangdi Protein Co., Ltd., specification: 50kg, the same below), adjust the pH of the feed solution to 7.0 with 30% sodium hydroxide solution, and stir at 40°C for 30min. Centrifuge at 4000g for 10min to remove bean dregs. Add 50 u / g of phytase calculated according to the protein content to the soybean protein feed liquid, and stir at 40°C for 30 minutes. After filtering the soybean protein material liquid through a 200-mesh filter, pour the filtrate into an ultrafiltration device with a molecular cut-off of 10,000 Daltons (SYNDER-UF101 ultrafiltration device, filter element size: Effective filter area: 0.1m 2 ), adjust the backflow control valve to control the ultrafiltration pressure between 0.1-0.15MPa. After the feed liquid is concentrated to 1 / 10 of the initial feed liquid volume, it is sterilized at 90°C for 15 seconds, and then spray-dried.

Embodiment 2

[0022] Add 3L of water to 300g of low-temperature defatted soybean meal, adjust the pH of the feed solution to 9.0 with 30% sodium hydroxide solution, stir at 50°C for 60min, and centrifuge at 4000g for 10min to remove the soybean dregs. Add 50 u / g of phytase calculated according to the protein content to the soybean protein feed liquid, and stir at 45°C for 30 minutes. After filtering the soybean protein material liquid through a 200-mesh filter, pour the filtrate into an ultrafiltration device with a molecular cut-off of 10,000 Daltons (SYNDER-UF101 ultrafiltration device, filter element size: Effective filter area: 0.1m 2 ), adjust the backflow control valve to control the ultrafiltration pressure between 0.1-0.15MPa. After the feed liquid is concentrated to 1 / 10 of the initial feed liquid volume, it is sterilized at 60° C. for 30 minutes, and then spray-dried.

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Abstract

The invention discloses a preparation method of acid soluble soy protein. The acid soluble soy protein is prepared by the steps of carrying out alkali dissolution to low temperature defatted soybean meal to obtain a soy protein solution, adding phytase, stirring, ultra-filtering, sterilizing and drying to obtain the acid soluble soy protein. The method is simple in steps and high in yield; and the acid soluble soy protein prepared by the method is remarkably high in acid solubility, transparency and mouthfeel.

Description

technical field [0001] The invention relates to a method for improving the solubility of soybean protein under acidic conditions, in particular to a method for improving the solubility of soybean protein under acidic conditions by combining phytase and ultrafiltration treatment. The obtained soybean Protein powder has high solubility and transparency in the acidic environment of pH2-4.5, and has a good taste at the same time. Background technique [0002] Soybean protein isolate is a plant protein with high nutritional value and superior functionality. Soybean protein isolate is widely used in meat products, dairy products, pasta and other fields. However, due to the low solubility of traditional soybean protein isolate in acidic environment, its application in acidic foods such as acidic drinks is limited. [0003] In order to increase the solubility of soybean protein under acidic conditions and fundamentally solve the problem of soybean protein application in acidic beve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/34
Inventor 高红亮黄橙子常忠义金明飞张亦澜谭静吕远崔红亮
Owner EAST CHINA NORMAL UNIV
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