Photostimulation method and photostimulation device
A technology of light stimulation and blue light emission, which is applied in the direction of light therapy, can solve the problems of inability to exert curative effect, inability to develop volume, small portable phototherapy device, etc., and achieve the effect of treating acne, inhibiting or killing acne bacillus
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Embodiment 1
[0039] refer to Figure 1 to Figure 3 ,in figure 1 It is a structural schematic diagram of the photostimulation device of the present invention, figure 2 It is a side view of the photostimulation device of the present invention, image 3 It is a system block diagram of the photostimulation device of the present invention.
[0040] Such as Figure 1 to Figure 3 As shown, the photostimulation device of the present invention includes: a casing 10 , a light-diffusing sheet 12 , a light-transmitting sheet 13 , a first light source module 40 , a second light source module 50 and a control module 30 .
[0041] The casing 10 forms an accommodating space for accommodating various modules. In addition, the casing 10 has a top surface 11 and a side edge 12 , the top surface 11 has a light outlet 111 , and the side edge 12 has a light hole 121 .
[0042] The light outlet 111 on the top surface 11 is covered by the diffuser 12 ; the light outlet 121 on the side edge 12 is covered by ...
Embodiment 2
[0048] Human fibroblasts were irradiated with the photostimulation device of Example 1 above, and its effect on cell viability was studied. In this embodiment, the light emitting diode used in the light source module of the light stimulation device emits red light with an illumination of 9,250 lux.
[0049] First, add DMEM cell culture medium containing human fibroblasts to a 48-well culture dish, and the number of cells inoculated is 2×10 4 Each well, the total volume of culture medium (including cells) in each well of 48 wells is 0.5ml, placed in CO 2 Incubator for 24 hours. After that, take out all the culture medium, add 0.5ml of PBS buffer, and use the red light stimulation device (Lux 9,250) of Example 1 to irradiate for 5, 10, 15, 30, 45, 60, 90 minutes, then take out all the culture medium in the well. Add 0.5 ml of culture medium to the PBS buffer and incubate for another 24 hours.
[0050] Then, replace with 0.5ml of new medium and add 0.125ml of MTT reagent, put ...
Embodiment 3
[0053] From the results of Example 2 above, it can be seen that after 5 minutes to 30 minutes of irradiation with a red light-emitting diode with an illumination of 9,250 lux, the survival rate of human fibroblasts can be improved, and all irradiation times are in line with the safety of the course of treatment. In this embodiment, the number of times of illumination was further changed from one to two, and the illumination of the red light-emitting diode was further reduced to 7,800 lux, and then the cell viability test was carried out.
[0054] First, add DMEM cell culture medium containing human fibroblasts to a 48-well culture dish, and the number of cells inoculated is 2×10 4 Each well, the total volume of culture medium (including cells) in each well of 48 wells is 0.5ml, placed in CO2 Incubator for 24 hours. Afterwards, take out all the culture medium, add 0.5 ml of PBS buffer, and use the red light stimulation device (Lux 7,800) of Example 1 to irradiate for 5, 10, 15,...
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