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Combined extraction method of grape seed oil, resveratrol and procyanidine in grape seeds

A technology of grape seed oil and resveratrol, which is applied in chemical instruments and methods, fat oil/fat production, organic compound preparation, etc., can solve the problems that it is not an extraction method and is difficult to realize, and achieves a short purification process, The effect of reducing production costs and less investment

Inactive Publication Date: 2015-06-03
GANSU AGRI UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires an additional ultrasonic process, which is difficult to achieve in factory production, and it is not an independent extraction method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 The combined extraction method of grapeseed oil, resveratrol and proanthocyanidins in grape seeds comprises the following steps:

[0018] ⑴Extraction of grape seed oil: Extract natural air-dried grape seeds with methanol at a material-to-liquid mass-volume ratio (g / ml) of 1:2 at a temperature of 20°C for 0.5 h each time to obtain crude grape seeds Seed oil and sediment A: Grape seed oil is obtained after the crude grape seed oil is decolorized through edible oil decolorizing clay OBT-40G column.

[0019] The grape seed oil is measured by weighing method, and its extraction rate is 3.1%.

[0020] (2) Extraction of resveratrol: Precipitate A was extracted once with ethyl acetate at a material-to-liquid mass-volume ratio (g / ml) of 1:5 at a temperature of 20°C for 2 hours each time to obtain resveratrol Crude resveratrol and precipitate B; crude resveratrol was extracted twice with equal volume of distilled water to obtain aqueous phase A and ethyl acetate org...

Embodiment 2

[0024] Example 2 The combined extraction method of grapeseed oil, resveratrol and proanthocyanidins in grape seeds comprises the following steps:

[0025] ⑴Extraction of grape seed oil: Extract natural air-dried grape seeds with methanol at a material-to-liquid mass-volume ratio (g / ml) of 1:10 at a temperature of 40°C for 3 times, each time for 4 hours, to obtain crude grape seeds Seed oil and sediment A: Grape seed oil is obtained after the crude grape seed oil is decolorized through edible oil decolorizing clay OBT-40G column.

[0026] The grape seed oil is measured by weighing method, and its extraction rate is 4.2%.

[0027] (2) Extraction of resveratrol: Precipitate A was extracted 4 times with ethyl acetate at a material-to-liquid mass-volume ratio (g / ml) of 1:30 at a temperature of 35°C for 12 hours each time to obtain resveratrol The crude product of resveratrol and precipitate B; the crude product of resveratrol was extracted by adding an equal volume of distille...

Embodiment 3

[0031] Example 3 The combined extraction method of grapeseed oil, resveratrol and proanthocyanidins in grape seeds comprises the following steps:

[0032] ⑴Extraction of grape seed oil: Extract natural air-dried grape seeds with methanol at a material-to-liquid mass-volume ratio (g / ml) of 1:6 at a temperature of 30°C twice for 2.5 hours each time to obtain crude grape seeds Seed oil and sediment A: Grape seed oil is obtained after the crude grape seed oil is decolorized through edible oil decolorizing clay OBT-40G column.

[0033] The grape seed oil is measured by weighing method, and its extraction rate is 3.4%.

[0034] (2) Extraction of resveratrol: Precipitate A was extracted three times with ethyl acetate at a material-to-liquid mass-volume ratio (g / ml) of 1:18 at a temperature of 25°C for 7 hours each time to obtain resveratrol The crude product of resveratrol and precipitate B; the crude product of resveratrol was extracted by adding an equal volume of distilled wa...

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PUM

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Abstract

The invention relates to a combined extraction method of grape seed oil, resveratrol and procyanidine in grape seeds. The method comprises the following steps of: (1) extraction of grape seed oil: extracting the naturally-dried grape seeds with methanol to obtain crude rape seed oil and a precipitate A respectively; and obtaining the grape seed oil after the crude grape seed oil flows through an edible oil decoloration argil OBT-40G column and is decolored; (2) extraction of resveratrol: extracting the precipitate A with ethyl acetate to obtain a crude product of resveratrol and a precipitate B respectively; and performing extraction, concentration, column chromatography and drying on the crude product of resveratrol according to a conventional technology to obtain resveratrol; and (3) extraction of procyanidine: extracting the precipitate B with alcohol with mass concentration of 70% to obtain a crude product of procyanidine and a precipitate C respectively; and performing column chromatography and drying on the crude product of procyanidine according to a conventional technology to obtain procyanidine. According to the combined extraction method provided by the invention, by adopting a co-production extraction mode, the active components in grape seeds are reasonably extracted one by one, and the production cost is effectively reduced.

Description

technical field [0001] The invention relates to an extraction method of active substances, in particular to a combined extraction method of grape seed oil, resveratrol and proanthocyanidins in grape seeds. Background technique [0002] At present, the extraction of active ingredients in grape pomace is basically the extraction of a single active substance. There are many extraction methods, most of which use modern biotechnology extraction methods, but none of them can carry out joint extraction of multiple active substances, and the waste of raw materials is serious. , low production efficiency, high cost, and serious environmental pollution. [0003] Extraction by supercritical fluid extraction: This method has expensive extraction equipment, high equipment precision requirements, low extraction rate, complex operation process, high operating cost, and joint extraction cannot be performed. [0004] Enzymatic extraction: the extraction rate is high, but the extraction cost...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C39/21C07C37/70C07D311/62C11B1/00
Inventor 李胜马绍英周文政李唯张真刘媛张品南刘会杰
Owner GANSU AGRI UNIV
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