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Screening method for deep-seeding-resisting corn germplasms

A screening method, corn technology, applied in botany equipment and methods, seed and rhizome treatment, soilless cultivation, etc., can solve the problems of increasing workload, affecting the accuracy of identification results, and single measurement indicators, so as to reduce work The effect of reducing the amount, shortening the screening time and improving efficiency

Active Publication Date: 2013-08-14
安徽英诺管理咨询有限公司
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Problems solved by technology

Although these deep-sowing-resistant treatment methods can screen out deep-sowing-resistant maize germplasm, the index of determination is single, which will affect the accuracy of identification results; in addition, when more maize germplasm materials are selected, the workload will be greatly increased.

Method used

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  • Screening method for deep-seeding-resisting corn germplasms
  • Screening method for deep-seeding-resisting corn germplasms
  • Screening method for deep-seeding-resisting corn germplasms

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Embodiment Construction

[0026] The methods used in the following examples, unless otherwise specified, are conventional methods; the equipment and reagents used are conventional reagents and equipment purchased by reagent companies.

[0027] 1. Collected materials: Collect various maize germplasm materials in various regions. The maize inbred lines are 45 listed in Table 1: namely 8723, TS141, 81162, and Liao 111.

[0028] 2. Seed cleaning: 90 plump and undamaged seeds were selected for each inbred line of 45 corn inbred lines.

[0029] 3. Pre-sowing preparation and sowing method: each inbred line corresponds to an experimental bucket with a height of 25cm and a diameter of 16cm. Evenly sow on the vermiculite in the experimental bucket, and then cover the vermiculite 15cm. The experiment was repeated 3 times.

[0030] 4. Experimental management: After the respective inbred lines are planted, they are placed in a solar greenhouse, the indoor temperature is kept at 25±2°C, and the humidity is...

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Abstract

The invention relates to a screening method for deep-seeding-resisting corn germplasms. The method comprises the following steps: collecting corn germplasm materials in areas; selecting 90 full and unbroken seeds from the corn germplasm materials; determining an experimental barrel which is 25cm in height and 16cm in diameter and corresponds to each variety, packaging vermiculites which are 10cm deep uniformly to all experimental barrels before seeding, and thoroughly pouring water; then, uniformly seeding 30 full and unbroken seeds selected from the selfing lines to vermiculites in the experimental barrels, then covering the vermiculites by 15cm, and repeating the experiment for 3 times; after seeding the germplasm materials, placing the germplasm materials in a same heliogreenhouse, maintaining the indoor temperature at 25+ / -2 DEG C and humidity at 70%+ / -3, and naturally germinating to emerge; in 10 days, carrying out statistic on rate of emergence, and retaining 10 strains which are consistent in growth for each sample to determine root, root length, mesocotyl length, coleoptile length, seedling length and the like; identifying and evaluating the deep-seeding resistances for the germplasms and screening the self lines with strong deep-seeding resistances. According to the method provided by the invention, the screening workload is reduced, and the screening efficiency and the accuracy of the screening result are improved.

Description

technical field [0001] The invention belongs to the technical field of breeding of new agricultural varieties, and in particular relates to a screening method of corn germplasm resistant to deep sowing. technical background [0002] During the germination and settlement stages of seedlings, lack of soil moisture is often the main cause of seedling death. Two-thirds of my country's maize area is distributed in the northeast, north, southwest and northwest hilly drylands or plain drylands that rely on natural rainfall. Drought has become an important limiting factor for high and stable maize yields. In the case of drought at the seedling stage, the emergence ability and seedling retention rate will decrease, which will seriously affect the number of seedlings in the field, resulting in a reduction in production. As a newly discovered germplasm resource that has been used to cultivate fine varieties, deep-sowing tolerant crops can effectively use deep soil moisture to avoid th...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01C1/00
Inventor 彭云玲闫慧萍赵小强
Owner 安徽英诺管理咨询有限公司
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