Application of icarisid II in preparation of sensitizer for chemotherapic medicine

A technology of icariin and chemotherapeutic drugs, applied in the field of biomedicine, can solve the problems of icariin II sensitization and the like, and achieve the effects of promoting drug sensitivity, reducing dosage and enhancing inhibitory effect.

Inactive Publication Date: 2013-08-14
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, so far, there has been no report on the sensiti

Method used

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  • Application of icarisid II in preparation of sensitizer for chemotherapic medicine
  • Application of icarisid II in preparation of sensitizer for chemotherapic medicine
  • Application of icarisid II in preparation of sensitizer for chemotherapic medicine

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Embodiment 1

[0026] 1. Materials and methods

[0027] 1. Cell line:

[0028] Human melanoma cell line (A375) and human skin squamous cell line (A431) were purchased from ATCC. The above cell lines were incubated in DMEM medium containing 10% fetal bovine serum at 37°C with a volume fraction of 5% CO 2 , Routine culture under fully saturated humidity conditions, replace the medium for 48 hours, when the cell growth reaches saturation, digest with 0.25% trypsin + 0.02% EDTA for passage, once every 2-3 days, the experiment uses logarithmic growth phase cells.

[0029] 2. Detection of cell proliferation:

[0030] Adjust the cell suspension concentration of A375 and A431 cell lines to 1×10 5 / mL, added to 96-well culture plate, 100 μL per well, placed at 37°C 5% CO 2 Incubator for 24h. Aspirate the old medium and culture with new DMEM medium containing 10% fetal bovine serum. Pre-incubated with icariside II at a final concentration of 10 μM for 1 hour, and then co-incubated with paclitaxe...

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Abstract

The invention belongs to the field of biological medicines, and relates to application of icarisid II in preparation of a sensitizer for a chemotherapic medicine. Shown in the experimental results, the icarisid II can obviously improve the inhibition effect of the taxol to the cancer cell proliferation, the taxol-induced tumor cell apoptosis, and the effect of inhibiting a TLR4 (Toll-Like Receptors 4) patch in order to improve the sensitivity of a chemotherapeutic medicine; the icarisid II can be used as the sensitizer for the chemotherapic medicine, and is applied to the tumor chemotherapy process, in order to obviously promote the medicine sensitivity of different tumor cells to the chemotherapic medicine, therefore, the dosage of the chemotherapic medicines is decreased, and the toxic and side effects of the chemotherapic medicine to normal cells of a human body are reduced. The icarisid II can be used as an active ingredient to be used together with a pharmaceutically acceptable carrier in order to prepare a pharmaceutical composition performing sensibilization in tumor chemotherapy, and the pharmaceutical composition is applied to chemosensitization for a plurality of tumors, including but not limited to melanoma and cutaneous squamous cell carcinoma and the like.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a new medicinal application of icariside II, an active ingredient of traditional Chinese medicine, in particular to the application of icariside II in preparing a tumor chemotherapeutic drug sensitizer. Background technique [0002] Chemotherapy is currently one of the main means of clinical treatment of tumors and some autoimmune diseases. In tumor treatment, high-concentration chemical drugs (sometimes called cytotoxic drugs) usually have a significant killing effect on tumor cells, but have relatively large toxic and side effects on normal tissues and organs, and patients generally have obvious side effects such as nausea and vomiting, resulting in Patients cannot tolerate it; while low-concentration chemotherapeutic drugs are well tolerated by patients, but their killing effect on tumor cells is often insufficient. Tumor chemotherapy sensitizer is a drug that can increase the sensit...

Claims

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Application Information

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IPC IPC(8): A61P35/00A61K31/7048
Inventor 徐金华董竞成吴金峰段晓虹秦海红朱小华
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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