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Chlorogenic acid and galuteolin in honeysuckle flower superhigh pressure extraction method and HPLC quantitative analysis method

A technology of luteolin and honeysuckle is applied in the biological field to achieve the effects of being beneficial to separation and purification, shortening extraction time and high compactness

Inactive Publication Date: 2013-08-21
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technique uses ultrasonics for faster and less expensive techniques than previous processes like distillation or evaporation. By doing it under very strong pressures, these techniques allow us to effectively separate chemical compounds from other materials without losing their original properties that are important for them being studied. Ultraviolet light absorption spectroscopy (UV), gas chromatography mass spectrometry(GCMS), liquid scintilation crystallography (LSCM), X ray diffraction scattering (XRSDS), infrared laser desorption ionization mass spectrum (IRIS). These technologies have been developed overall, providing technical benefits such improved process efficacy and reduced costs compared to older methods while also improving productivity.

Problems solved by technology

This patents describes how it can be useful when preparing medicine from plants called lilies that contain flavonoids like lycopene (L) and quinolones). These compounds help fight diseases caused by harmful bile pills. However, current techniques require expensive processes involving multiple steps, resulting in low yields due to incomplete extractions.

Method used

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  • Chlorogenic acid and galuteolin in honeysuckle flower superhigh pressure extraction method and HPLC quantitative analysis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: different solvent experiments

[0022] Grind the dried honeysuckle through a 60-80 mesh sieve, accurately weigh 4 parts of 1.0g dried honeysuckle powder, put them in plastic sealed bags, add 50ml of water, 70% ethanol, methanol, n-butanol, seal and soak for 24 Hour. Then put it into an ultra-high pressure equipment, pressurize 300MPa for leaching, extract at a temperature of 30°C, hold the pressure for 5 minutes, release the pressure, take 2ml of the supernatant in a 10ml volumetric flask after centrifugation, use distilled water to make up the volume, and use the HPLC method to extract Determination of chlorogenic acid and luteolin content. HPLC conditions are: Cosmosil π-NAP C 18 Chromatographic column (5 μm, 4.6 mm × 250 mm); mobile phase: A: B = acetonitrile: 0.4% phosphoric acid solution; gradient elution conditions: 0-15min, B: 90-80%, 15-30min, B: 80 %, 30-40min, B: 80-70%; column temperature: 30°C; flow rate: 1ml / min; detection wavelength: 327n...

Embodiment 2

[0026] Embodiment 2: solvent concentration test

[0027] Crush the dried honeysuckle through a 60-80 mesh sieve, accurately weigh 5 parts of 1.0g dried honeysuckle powder, put them in plastic sealing bags, and add 50ml of 10%, 30%, 50%, 70% and 90% ethanol respectively , sealed and soaked for 24 hours. Then put it into an ultra-high pressure equipment, pressurize 300MPa for leaching, extract at a temperature of 30°C, hold the pressure for 5 minutes, release the pressure, take 2ml of the supernatant in a 10ml volumetric flask after centrifugation, use distilled water to make up the volume, and use the HPLC method to extract Determination of chlorogenic acid and luteolin content. HPLC conditions are: Cosmosil π-NAP C 18 Chromatographic column (5 μm, 4.6 mm × 250 mm); mobile phase: A: B = acetonitrile: 0.4% phosphoric acid solution; gradient elution conditions: 0-15min, B: 90-80%, 15-30min, B: 80 %, 30-40min, B: 80-70%; column temperature: 30°C; flow rate: 1ml / min; detection ...

Embodiment 3

[0031] Embodiment 3: Comparison test of different extraction pressures

[0032] Crush the dried honeysuckle through a 60-80 mesh sieve, accurately weigh 5 parts of 1.0g dried honeysuckle powder, put them in plastic sealed bags, add 50ml of 70% ethanol respectively, seal and soak for 24 hours. Then put it into the ultra-high pressure equipment, pressurize 100, 200, 300, 400, 500MPa respectively for leaching, extract at 30°C, hold the pressure for 5min, release the pressure, take 2ml of the supernatant in a 10ml volumetric flask after centrifugation, Distilled water was used to make up the volume, and the content of chlorogenic acid and luteolin was determined by HPLC method. HPLC conditions are: Cosmosil π-NAP C 18 Chromatographic column (5 μm, 4.6 mm × 250 mm); mobile phase: A: B = acetonitrile: 0.4% phosphoric acid solution; gradient elution conditions: 0-15min, B: 90-80%, 15-30min, B: 80 %, 30-40min, B: 80-70%; column temperature: 30°C; flow rate: 1ml / min; detection wavel...

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Abstract

The invention discloses a rapid and high efficiency chlorogenic acid and galuteolin in honeysuckle flower extraction method and a HPLC quantitative analysis method, and which comprise the following steps: superhigh pressure extraction and quantitative analysis. Superhigh pressure extraction comprises the steps as follows: firstly crushing dried honeysuckle flower and filtering by a sieve with 60-80 meshes, adding water or ethanol, methanol and n butanol organic solvent into the honeysuckle flower powder according to 1:10-1:100 solid liquid ratio, mixing and sealing, immersing at normal temperature, placing the mixture into a high pressure container, leaching with pressure at 20-60 DEG C. for 1-5 min, relieving pressure, carrying out centrifugation of extract product and obtaining the honeysuckle flower extract. Quantitative analysis: after centrifugation, diluting 2ml of the obtained supernatant to 10 ml with distilled water in a volumetric flask, refrigerating for standby. The chlorogenic acid and galuteolin in honeysuckle flower are extracted by a superhigh pressure technology and are carried out by a content determination, and the invention has the characteristics of high recovery rate, short extraction time, small solvent amount, simple operation, accuracy, good repeatability, no pollution, safety, low energy consumption, etc.

Description

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Claims

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Application Information

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Owner SUZHOU UNIV
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