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Preparation method and application of eggplant branch polysaccharide

A polysaccharide and eggplant branch technology, applied in the field of plant polysaccharide preparation, can solve the problems of precious and scarce plant polysaccharide raw materials, insufficient extraction technology, high production cost, etc., and achieve the effects of improving immunity, simple process and low cost

Active Publication Date: 2013-09-04
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of rare raw materials of plant polysaccharides, and propose a method for extracting polysaccharides from eggplant rhizomes that can solve the problem of high production costs and insufficient extraction techniques

Method used

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  • Preparation method and application of eggplant branch polysaccharide
  • Preparation method and application of eggplant branch polysaccharide
  • Preparation method and application of eggplant branch polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method of eggplant branch polysaccharide, the steps are as follows:

[0028] (1) Pretreatment: Wash the eggplant rhizomes, dry them, crush them, and pass through a 60-mesh sieve to obtain eggplant rhizome powder;

[0029] (2) Ethanol degreasing: Take 500g of eggplant rhizome powder, add 95% ethanol with 10 times the volume of eggplant rhizome powder, heat and reflux for 2 hours, discard the ethanol solvent; add 95% ethanol with 10 times the volume of the residue again, and heat and reflux for 2 hours , the ethanol solvent was discarded, the remaining residue was washed to remove ethanol, and then air-dried naturally to obtain degreased eggplant rhizome powder, which was set aside;

[0030] (3) Extraction: Take the degreased eggplant rhizome powder, add distilled water according to the ratio of solid to liquid 1:80 (the ratio of solid to liquid is, 1g of degreased eggplant rhizome powder is added to 80ml of distilled water), extract at 60°C for 2 hours, and...

Embodiment 2

[0035] (1) Detection

[0036] The solanaceae polysaccharide after the separation and purification obtained in Example 1 is fully hydrolyzed with the concentrated sulfuric acid of 1mol / L, BaCO 3 Neutralize to neutral, concentrate, filter, and centrifuge to take the supernatant to prepare isolated and purified Solanum polysaccharide samples. The same operation is performed to prepare mannose, galactose, glucose, arabinose, xylose, and fructose monosaccharide standard products.

[0037] For thin-layer chromatography, pre-column derivatization of the separated and purified eggplant branch polysaccharide samples and six kinds of monosaccharide standard products was carried out, and high-performance liquid chromatography analysis was performed, and the retention time was recorded. Through thin-layer chromatography and high-performance liquid chromatography Method analysis proves that the isolated and purified solanum polysaccharide prepared by the method of the present invention is ...

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Abstract

The invention relates to a preparation method of eggplant branch polysaccharide. The adopted technical scheme is as follows: the preparation method comprises the following steps of: pre-treating the rootstocks of eggplants, degreasing by using ethanol, then adding distilled water, extracting at the temperature of 60 DEG C for 2h, and carrying out ethanol precipitation to obtain a crude product of the eggplant branch polysaccharide; removing proteins by using an enzymolysis and trichloroacetic acid reagent combination method to obtain purified eggplant branch polysaccharide; and dissolving the purified eggplant branch polysaccharide into bi-distilling water, and separating and purifying a polysaccharide mixture by using a DEAE-D22 cellulous column and a Sephadex G-100 column to obtain separated and purified eggplant branch polysaccharide with relatively single component. By using the preparation method, the problems of rare plant polysaccharide raw materials, high production cost and insufficient extraction process are solved, the raw materials are simple and easy to obtain, the optimal extraction process is designed, the finally obtained eggplant branch polysaccharide has the biological activities of oxidation resistance and immunity improvement, and novel raw materials and production processes are provided for applying the eggplant branch polysaccharide to the food and medicine production fields.

Description

technical field [0001] The invention relates to a method for preparing plant polysaccharides, in particular to a method for extracting eggplant branch polysaccharides from eggplant rhizomes. Background technique [0002] At present, there are more than 300 known natural polysaccharide biopolymers. Due to the complexity of polysaccharides and their complexes, compared with proteins and nucleic acids, the research on polysaccharides in my country started late, and the degree of attention is not enough. However, with the rapid development of biochemistry in recent years, the analysis of the structure and biological activity of polysaccharides has become more and more in-depth, and it has gradually been found that polysaccharides have great utilization value for the human body. In recent years, a large number of experimental results have shown that plant polysaccharides have physiological functions such as regulating immunity, anti-oxidation, antibacterial, anti-radiation, anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 王秋雨金莉莉吕磊
Owner LIAONING UNIVERSITY
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