Cell impedance sensor apparatus for detecting marine aquatic product diarrheal toxin, and method
A technology for aquatic products and sensor chips, applied in the direction of material impedance, etc., to achieve the effect of non-destructive cells, simple operation steps and good repeatability
Inactive Publication Date: 2015-06-17
ZHEJIANG UNIV
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However, there is no literature report on the use of cell impedance sensors to detect diarrheal toxins in marine aquatic products.
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[0017] The following describes the present invention in detail with reference to the drawings and specific embodiments, but it does not limit the present invention.
[0018] Such as Figure 1-2 As shown, the cell impedance sensor detection device of the present invention includes: an environmental chamber, a chip mounting table, a single-chip microcomputer 14, a temperature sensor 3, a cell impedance sensor chip 5, and a heater 10. The environmental chamber is composed of an environmental chamber base 1, a first environmental chamber positioning button 2, a second environmental chamber positioning button 11, and an environmental chamber top cover 7. The environmental chamber top cover 7 passes through the first environmental chamber positioning button 2 and the second environment The chamber positioning buckle 11 is fixed on the base 1 of the environmental chamber to form a closed environment. The chip mounting table, heater 10 and temperature sensor 3 are all fixed in the envir...
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The present invention discloses a cell impedance sensor apparatus for detecting marine aquatic product diarrheal toxin, and a method. The apparatus comprises an environment chamber, a chip installation table, a single chip microcomputer, a temperature sensor, a cell impedance sensor chip and a heater. The method comprises that: a marine aquatic product diarrheal toxin solution sample is prepared, the single chip microcomputer adopts a PID control algorithm to control the heater to achieve 37 DEG C ambient temperature thermostatic control, a sensor signal calculation baseline value is received from the cell impedance sensor chip, a CI index algorithm is adopted to calculate a cell impedance sensor chip signal CI value, a CI-time curve is obtained through sample solution detection, and comparison of the CI-time curve and a standard diagram of the cell impedance sensor chip is performed to obtain a concentration interval of the first sample solution so as to obtain marine aquatic product diarrheal toxin content. With the present invention, semi-quantitative detection on marine aquatic product diarrheal toxin is achieved, and characteristics of high throughput, high sensitivity, good reproducibility and the like are provided.
Description
Technical field [0001] The invention relates to a detection technology for marine aquatic product diarrhea toxins, in particular to a cell impedance sensor device and method for detecting marine aquatic product diarrhea toxins. Background technique [0002] Diarrheal toxins of marine aquatic products are formed by the accumulation of harmful organic substances collected by shellfish from planktonic algae. They are a class of highly toxic non-protein toxins that can poison people and even die after consumption. At present, the domestic marine aquatic product diarrheal toxin detection method usually uses qualitative or semi-quantitative mouse biological detection method, but this method has low detection sensitivity, cannot determine the toxin structure in the sample, and the operation is cumbersome and time-consuming and difficult to promote. The cell impedance sensor calculates the cell index by detecting the magnitude of impedance to characterize cell attachment, morphological c...
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IPC IPC(8): G01N27/02
Inventor 王平苏凯麒胡宁王琴邹玲
Owner ZHEJIANG UNIV