Solid fermentation process of golden flower black tea
A golden flower black tea, solid fermentation technology, applied in the field of black tea, to achieve the effect of ensuring the quality of flowering, shortening the flowering period, and eliminating the possibility of contamination of dark tea by bacteria
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Embodiment 1
[0019] 1. Identify the strains of Eurotium cristatum cristatum, compare the colony morphology and aroma after fermentation; and compare it with Eurotium cristatum gene for 18S rRNA partial sequence, strain: WB 4222) sequence comparison, the comparison sequence requires more than 1500bp, the similarity is 99.9%, as a bacterial species
[0020] 2. Dark tea charging: place the dark tea obtained according to the traditional dark tea production process in a 50-liter solid fermentation tank, and the charging volume is 60% of the volume of the fermentation tank
[0021] 3. Dark tea steaming: Steam sterilization, heat up to 90°C and steam for 30 minutes, during the sterilization process, the rotation speed of the solid fermenter is 200 rpm
[0022] 4. Vaccination
[0023] 4.1. Preparation of inoculum solution: prepare dark tea juice with a concentration of 0.8% in purified water, add glucose with a concentration of 6% in the tea juice, and sterilize by autoclaving
[0024] 4.2. Unde...
Embodiment 2
[0028] 1. Identify the strains of Eurotium cristatum cristatum, compare the colony morphology and aroma after fermentation; and compare it with Eurotium cristatum gene for 18S rRNA partial sequence, strain: WB 4222) sequence comparison, the comparison sequence requires more than 1500bp, the similarity is 99.9%, as a bacterial species
[0029] 2. Dark tea charging: place the dark tea obtained by processing according to the traditional dark tea production process in a 1 ton solid fermenter, and the charging volume is 80% of the fermenter volume
[0030] 3. Dark tea steaming: Steam sterilization, heat up to 130°C and steam for 10 minutes, during the sterilization process, the rotation speed of the solid fermenter is 200 rpm
[0031] 4. Vaccination
[0032] 4.1. Preparation of inoculum solution: prepare dark tea juice with a concentration of 10% in pure water, add glucose with a concentration of 20% in the tea juice, and sterilize by autoclaving
[0033] 4.2. Under aseptic condi...
Embodiment 3
[0037] 1. Identify the strains of Eurotium cristatum cristatum, compare the colony morphology and aroma after fermentation; and compare it with Eurotium cristatum gene for 18S rRNA partial sequence, strain: WB 4222) sequence comparison, the comparison sequence requires more than 1500bp, the similarity is 99.9%, as a bacterial species
[0038] 2. Dark tea charging: put the black tea powder (particle size 100um) pulverized by a pulverizer into a 100-liter solid fermentation tank, and the charging volume is 80% of the volume of the fermentation tank. %
[0039] 3. Black tea steaming: Steam sterilization, heat up to 110°C and steam for 20 minutes, during the sterilization process, the rotation speed of the solid fermentation tank is 200 rpm
[0040] 4. Vaccination
[0041] 4.1. Preparation of inoculum solution: prepare black tea juice with a concentration of 1% in purified water, add sucrose with a concentration of 6% in the tea juice, and sterilize by autoclaving
[0042] 4.2. U...
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